AM33270PU-S pan Cytokeratin antibody

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0.1 mg / €320.00
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Quick Overview

Mouse anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat pan Cytokeratin SPM115 + SPM116

Product Description for pan Cytokeratin

Mouse anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat pan Cytokeratin SPM115 + SPM116.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for pan Cytokeratin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Cytokeratin pan-reactive, pan Keratin
Presentation Purified
Reactivity Bov, Can, Chk, Hu, Mky, Ms, Rb, Rt
Applications F, ICC/IF, P, WB
Clonality Monoclonal
Clone SPM115 + SPM116
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human epidermal keratin.
Isotype control AM03095PU-N
Application Western Blot: 0.5-1 µg/ml. 
Flow Cytometry:
0.5-1 µg/106 cells.
Immunofluorescence: 1-2 µg/ml.
Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. 
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: Skin, Adeno- or Squamous carcinomas.
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).
Concentration 0.2 mg/ml
General Readings
  1. Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8. PubMed PMID: 6183275. (Free PMC Article available)
  2. Tseng SCG et. al. Cell 1982; 30361.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Species Reactivity
Species reactivity (tested):
Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken.
Twenty Human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI < 5.7) and basic (pI > 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
This Monoclonal Antibody is a broad spectrum anti pan-Cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms.
This antibody stains Cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Cellular Localization: Cytoplasmic.

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