BM5001 pan Cytokeratin antibody

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Mouse anti Bovine, Chicken, Human, Mouse, Rabbit, Rat pan Cytokeratin AE1+AE3


Product Description for pan Cytokeratin

Mouse anti Bovine, Chicken, Human, Mouse, Rabbit, Rat pan Cytokeratin AE1+AE3.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for pan Cytokeratin

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Bov, Chk, Hu, Ms, Rb, Rt
Applications C, P, WB
Clonality Monoclonal
Clone AE1+AE3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human epidermal keratin
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Immunohistochemistry on frozen and paraffin embedded sections and cytological material: dilute 1/100 - 1/200 in PBS, pH 7.4. Incubation time: 2h at 37°C (or overnight at 2-8°C).
With paraffin-embedded sections, protease pretreatment is recommended prior to antibody application.
Western blotting.
Concentration 1 mg/ml
General Readings Battifora, H. et al. Cancer 54, 843-848 (1984)
Eichner, R., et al. J. Cell Biol. 98, 1388-1396 (1984)
Garin-Chesa, P. et al. Am J. Surg. Pathol. 10, 829-835 (1986)
Nelson, W. G. et al. Cancer Res. 44, 1600-1603 (1984)
Nelson, W.G. et al. J. Cell Biol. 97, 244-251 (1983)
Moll, R., et al. Cell 31, 11-24 (1982)
Shi, S. R. et al. Am J. Pathol. 117, 53-63 (1984)
Spagnolo, D. V. et al. Am. J. Clin. Pathol. 84, 697-704 (1985)
Sun, T. T. et al. The Cancer Cell 1, 169-176 (1984)
Sun, T. T. et al. J. Invest. Dermatol. 81, 109-115 (1983)
Sun, T. T. et al. The New York Acad. Sci. (E. Wang et al. eds.) 455, 307-329 (1985)
Tseng, S. C. G. et al. Cell 30, 361-372 (1982)
Woodcock-Mitchell, J. et al. J. Cell Biol. 95, 580-588 (1982)
Weiss, R. A. et al. J. Cell Biol. 98, 1397-1406 (1984)
Storage Store the antibody at 2-8°C. Shelf life: one year from despatch.
Affinity chromatography purified antibody containing 0.09% sodium azide
AE1/AE3 represents an excellent marker for distinguishing carcinomas from non-eptithelial tumors; reacts with all epithelium-derived tumors and their neoplasm. Decorates the majority of type I and neoplasms. Decorates the majority of type I and type II cytokeratins. Polypeptides reacting: Mr 40 000, Mr 48 000 and Mr 50 000 polypeptides of human epithelial proteins (cytokeratins nos. 19, 16, 14) and most other acidic (type I) cytokeratins (AE1); most basic (type II) cytokeratins (Mr 52 500 - 68 000) of human epithelial (AE3).
human, rabbit, mouse, rat, bovine, chicken, monkey and guinea pig.

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