R1103P Pancreatic Islet Lysate antibody

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0.5 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Dog anti Porcine Pancreatic Islet Lysate

R1103P

Product Description for Pancreatic Islet Lysate

Dog anti Porcine Pancreatic Islet Lysate.
Presentation: Ig Fraction
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Pancreatic Islet Lysate

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Ig Fraction
Reactivity Por
Applications E, WB
Clonality Polyclonal
Host Dog
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.
Application Suitable for Immunoblotting (Western or Dot blot) and ELISA.
Recommended Dilutions: This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by Immunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Kühtreiber WM, Lanza RP, Chick WL. Secretory function of biohybrid pancreas devices containing isolated porcine islets. ASAIO J. 1994 Jul-Sep;40(3):M789-92. PubMed PMID: 8555622.
  2. Warnock, C.L., Rajotte, R.V. (1988) Critical Mass of Purified Islet that Induce Implantation into Diabetic Dogs. Diabetes 37; 467-470.
Storage Store vial at -70°C prior to opening.
For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Purification:
Multi-step process.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
State:
Liquid (sterile filtered) purified Ig fraction.
Ig Fraction
Specificity
Specificity:
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum.
Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates.

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