AM10035FC-N PCNA antibody

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0.1 mg / €400.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Amphibian, Drosophila, Fish, Mammalia, Yeast PCNA PC10

Product Description for PCNA

Mouse anti Amphibian, Drosophila, Fish, Mammalia, Yeast PCNA PC10.
Presentation: FITC
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for PCNA

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Cyclin, Proliferating Cell Nuclear Antigen
Presentation FITC
Reactivity Amph, Dros, Fi, Mam, Ye
Applications F, ICC/IF
Clonality Monoclonal
Clone PC10
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P12004
Immunogen:
Rat PCNA made in the protein A expression vector pR1T2T.
GeneID:
5111
Application Flow Cytometry: Use 10 µl of Neat-1/10 of dilutued antibnody to label 106 cells in 100 µl. Cell permeabilisation is required for this application.
Background Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle It is increased during late G1 phase and S phase of the cell cycle and declines during G2 and M phases.
Concentration 0.1 mg/ml
General Readings
  1. Mathews MB, Bernstein RM, Franza BR, Garrels JI. Identity of the proliferating cell nuclear antigen and cyclin. Nature. 1984 May 24-30;309(5966):374-6. PubMed PMID: 6145097.
  2. Garcia RL, Coltrera MD, Gown AM. Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis. Am J Pathol. 1989 Apr;134(4):733-9. PubMed PMID: 2565087. (Free PMC Article available)
  3. Landberg G, Tan EM, Roos G. Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle. Exp Cell Res. 1990 Mar;187(1):111-8. PubMed PMID: 1967582.
  4. Wilson, G.D. et al. (1992) Flow cytometric characterisation of proliferating cell nuclear antigen using monoclonal antibody PC1O. Eur. J. Cancer 28A: 2010-2017.
  5. Elsässer HP, Biederbick A, Kern HF. Growth of rat pancreatic acinar cells quantitated with a monoclonal antibody against the proliferating cell nuclear antigen. Cell Tissue Res. 1994 Jun;276(3):603-9. PubMed PMID: 7914831.
  6. Prosperi E, Stivala LA, Sala E, Scovassi AI, Bianchi L. Proliferating cell nuclear antigen complex formation induced by ultraviolet irradiation in human quiescent fibroblasts as detected by immunostaining and flow cytometry. Exp Cell Res. 1993 Apr;205(2):320-5. PubMed PMID: 8097724.
  7. Harris, L. et al. (2006) Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques Aquaculture 261: 1413-21
  8. Buggins AG, Milojkovic D, Arno MJ, Lea NC, Mufti GJ, Thomas NS, et al. Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways. J Immunol. 2001 Nov 15;167(10):6021-30. PubMed PMID: 11698483.
  9. Kapitonova MY, Kuznetsov SL, Khlebnikov VV, Zagrebin VL, Morozova ZCh, Degtyar YV. Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress. Neurosci Behav Physiol. 2010 Jan;40(1):97-102. doi: 10.1007/s11055-009-9217-4. Epub 2009 Dec 11. PubMed PMID: 20012496.
  10. Franz-Odendaal TA. Toward understanding the development of scleral ossicles in the chicken, Gallus gallus. Dev Dyn. 2008 Nov;237(11):3240-51. doi: 10.1002/dvdy.21754. PubMed PMID: 18855894.
  11. Hashimoto Y, Ray Chaudhuri A, Lopes M, Costanzo V. Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis. Nat Struct Mol Biol. 2010 Nov;17(11):1305-11. doi: 10.1038/nsmb.1927. Epub 2010 Oct 10. PubMed PMID: 20935632. (Free PMC Article available, 6 images available)
  12. Jenkins H, Hölman T, Lyon C, Lane B, Stick R, Hutchison C. Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix. J Cell Sci. 1993 Sep;106 ( Pt 1):275-85. PubMed PMID: 7903671.
  13. Park JH, Seok SH, Baek MW, Lee HY, Kim DJ, Park JH. Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice. J Comp Pathol. 2008 Nov;139(4):208-17. doi: 10.1016/j.jcpa.2008.04.005. Epub 2008 Sep 26. PubMed PMID: 18823636.
  14. Izhak L, Wildbaum G, Jung S, Stein A, Shaked Y, Karin N. Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis. PLoS One. 2012;7(1):e28305. doi: 10.1371/journal.pone.0028305. Epub 2012 Jan 18. PubMed PMID: 22279523. (Free PMC Article available, 4 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light. 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction
Specificity
Specificity:
This antibody recognises the proliferating cell nuclear antigen (PCNA), a nuclear protein vital for cellular DNA synthesis. 
PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti Human PCNA, clone PC10 has been used for the detection of PCNA in a number of species including Rat (Elsässer et al. 1994), Mouse (Park et al. 2008), Chicken (Franz-Odendaal 2008) and Abalone (Harris et al. 2006).
Species:
Human, Insects, Vertebrates, Rat, Ferret, Chicken, Rabbit, Xenopus, Rhesus Monkey, Hamster, Atlantic Salmon, Mouse, Horse, Sheep, Dog, Cat, Cynomolgus monkey.



FocusOn and Reviews
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Accessory Products

Proteins and/or Positive Controls

Proteins for PCNA (18 products)

Catalog No. Species Pres. Purity   Source  

PCNA (transcript variant 1)

PCNA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

PCNA (full length, N-term HIS tag, transcript variant 2)

PCNA Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

PCNA

PCNA Human Purified >90.0% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

PCNA

PCNA Human Purified > 90 % >90.0% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

PCNA (1-261)

PCNA Human Purified > 95 % by SDS PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

PCNA (1-261)

PCNA Human Purified > 95 % by SDS PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

PCNA

PCNA Human Purified > 90 % E. coli
  OriGene Technologies GmbH

PCNA

PCNA Human Purified > 90 % E. coli
  OriGene Technologies GmbH

PCNA

PCNA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PCNA

PCNA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PCNA

PCNA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PCNA

PCNA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PCNA

PCNA Human > 98 % E. coli
  Funakoshi Co., Ltd.

PCNA

PCNA Human > 98 % E. coli
  Funakoshi Co., Ltd.

PCNA

PCNA Human Purified > 90 % SF9 cells
  Neuromics Antibodies

PCNA

PCNA Human Purified > 90 % SF9 cells
  Neuromics Antibodies

PCNA

PCNA Human Purified > 90 % SF9 cells
  Neuromics Antibodies

PCNA

SDS-Page: PCNA Protein [NBC1-18428] - PCNA, 28 kDa (261 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for PCNA (4 products)

Catalog No. Species Pres. Purity   Source  

PCNA Lysate

Western Blot: PCNA Lysate [NBL1-14184] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PCNA
  Novus Biologicals Inc.

PCNA overexpression lysate

PCNA overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

PCNA overexpression lysate

PCNA overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

PCNA overexpression lysate

PCNA overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.
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