DM3171 Pds1 / Securin antibody

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Mouse anti Human Pds1 / Securin DCS-280


Product Description for Pds1 / Securin

Mouse anti Human Pds1 / Securin DCS-280.
Presentation: Purified
Product is tested for Paraffin Sections.

Properties for Pds1 / Securin

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications P
Clonality Monoclonal
Clone DCS-280
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Human recombinant full length Pds1 protein.
Isotype control AM03096PU-N
Application Western Blotting (Not verified). Immunohistology (formalin/paraffin): Ab 1-2 micrograms/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: HeLa Cells, Tonsil. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Wang H, et al. (2001) Gen
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Pds1 is an anaphase inhibitor and plays important role in DNA damage and spindle check point pathways. Pds1 inhibits sister chromatid separation by binding and inhibiting Esp1, cystein protease that causes cleavage of the cohesion Scc1 that binds the sister chromatids together. Pds1 is responsible for targeting of Esp1 to nucleus and its binding to the spindle. Degradation of Pds1 occurs shortly before anaphase, which liberates Esp1 and is a prerequisite for anaphase entry. Pds1 is targeted for degradation by ubiquitination mediated by cyclosome/anaphase-promoting complex (APC) functioning as a ubiquitin ligase. In response to DNA damage Chk1 phosphorylates Pds1 to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase. Cellular Localization: nuclear.

Mol. Wt of Antigen: 42kD

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