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R1532P Peptide Transporter 1 (PepT1) antibody

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0.1 mg / €440.00

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Rabbit anti Human Peptide Transporter 1 (PepT1)

R1532P

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Product Description for Peptide Transporter 1 (PepT1)

Rabbit anti Human Peptide Transporter 1 (PepT1).
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for Peptide Transporter 1 (PepT1)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Reactivity Hu
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide C-T-M-S-G-K-I-G-A-L-E-I corresponding to human PepT1 conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Application Affinity purified rabbit anti-PepT1 has been tested by ELISA, immunohistochemistry and western blotting against both the immunizing peptide, native and recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and other immunodetection techniques.
This product was assayed by immunoblot and was found to be reactive at 1:2,000 with extracts from human liver and small intestine.
Strong immunoreactivity was observed in similar tissue when stained for immunohistochemistry using a 1:2,000 dilution of the antibody.
Background Peptide Transporter 1 (PepT1) is an oligopeptide transporter expressed at the brush-border membrane of intestinal epithelial cells and has a predominant role in intestinal absorption of natural di- and tripeptides and peptide-based drugs. PepT1 is localized predominantly in the upper small intestine, with decreasing expression in the ileum. PepT1 is a H(+)-coupled transporter that is able to transport oligopeptides and peptide-derived antibiotics through the small intestine. PepT1-mediated uptake is independent of extracellular Na+, K+ and Cl-, and of membrane potential. PepT1 displays low affinity and unusually broad substrate specificity. Consequently, PepT1 accepts various peptide mimetic drugs such as β- lactam antibiotics, angiotensin converting enzyme inhibitors, renin inhibitors and anticancer drugs. PepT1 can be exploited for improvement of intestinal absorption of poorly absorbed drugs by derivation of the drugs to peptide mimetics. Such strategies have been tested across the membranes of human intestinal epithelial-like Caco-2 cells and in certain tumor cells. PepT1 may improve delivery of peptide-mimetic anticancer drugs to tumors by utilization of this peptide transporter activity. Lys-Xaa dipeptides and derivatives have been shown to be an effective competitive inhibitor of mammalian PepT1 in Caco-2 cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings C.J. Sword, A.A. Constantinou, I.D. Collier, P.D. Bailey and C.A.R. Boyd (2000) Modified dipeptides inhibit substrate uptake into PepT1-expressing Xenopus oocytes. J. Physiology: 528P, 99.
Fei, Y.J., Kanai, Y., Nussberger, S., Ganapathy, V., Leibach, F.H., Romero, M.F., Singh, S.K., Boron, W.F. & Hediger, M.A. (1994). Expression cloning of a mammalian proton-coupled oligopeptide transporter. Nature 368, 563-566.
D.R. Herrera, Q. Wang, O.S. Gudmundsson, T. Cook, R. Smith, T.N. Faria, and G.T. Knipp. (2001) Spatial Expression Patterns of Peptide Transporters in the Human and Rat Gastrointestinal Tracts, Caco-2 in vitro Cell Culture, and Multiple Human Tissues. AAPS PharmSci. 3(1):9.
D.R. Herrera and G.T. Knipp (2003). Current Perspectives on Established and Putative Mammalian Oligopeptide Transporters. J. Pharm. Sci. 92:000-000.
Storage Store (in aliquots) at -20°C or below.
Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Purification:
Affinity purified from whole rabbit serum
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative
State:
Liquid (sterile filtered) purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This antibody is directed against human PepT1 and is useful in determining its presence in various assays. Anti-PepT1 antibody detects endogenous and overexpressed proteins found in various tissues and extracts.
Reactivity is observed against human PepT1 and cross reactivity with PepT1 from other mammalian sources has not been tested.

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