DP2019 Periostin antibody

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50 µg / €400.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Chicken, Human, Mouse, Rat Periostin

DP2019

Product Description for Periostin

Rabbit anti Chicken, Human, Mouse, Rat Periostin.
Presentation: Azide Free
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for Periostin

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms OSF-2, OSF2, Osteoblast-specific factor 2, PN, POSTN
Presentation Azide Free
Reactivity Chk, Hu, Ms, Rt
Applications C, E, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q15063
Immunogen:
Recombinant Human OSF-2
Source of Antigen: E. coli.
AA Sequence:
MGHHHHHHHH HHSSGHIEGR HMRNNHYDKI LAHSRIRGRD QGPNVCALQQ ILGTKKKYFS TCKNWYKKSI CGQKTTVLYE CCPGYMRMEG MKGCPAVLPI DHVYGTLGIV GATTTQRYSD ASKLREEIEG KGSFTYFAPS NEAWDNLDSD IRRGLESNVN VELLNALHSH MINKRMLTKD LKNGMIIPSM YNNLGLFINH YPNGVVTVNC ARIIHGNQIA TNGVVHVIDR VLTQIGTSIQ DFIEAEDDLS SFRAAAITSD ILEALGRDGH FTLFAPTNEA FEKLPRGVLE RFMGDKVASE ALMKYHILNT LQCSESIMGG AVFETLEGNT IEIGCDGDSI TVNGIKMVNK KDIVTNNGVI HLIDQVLIPD SAKQVIELAG KQQTTFTDLV AQLGLASALR PDGEYTLLAP VNNAFSDDTL SMVQRLLKLI LQNHILKVKV GLNELYNGQI LETIGGKQLR VFVYRTAVCI ENSCMEKGSK QGRNGAIHIF REIIKPAEKS LHEKLKQDKR FSTFLSLLEA ADLKELLTQP GDWTLFVPTN DAFKGMTSEE KEILIRDKNA LQNIILYHLT PGVFIGKGFE PGVTNILKTT QGSKIFLKEV NDTLLVNELK SKESDIMTTN GVIHVVDKLL YPADTPVGND QLLEILNKLI KYIQIKFVRG STFKEIPVTV Y
GeneID:
10631
Remarks:
The immunization antigen (75 kDa) is a protein containing 648 amino acids of recombinant Human OSF-2 and 23 amino acids, N-Terminal HisTag and Xa - cleavage site (highlighted). The antigen contains amino acid residues 22 to 669 of the Human OSF-2 precursor.
The antigen includes all four fasciclin domains of OSF-2.
Add. information

Antibody Content
0.05 mg (determined by BCA method, BSA was used as a standard). 

Quality Control Test
Indirect ELISA - to determine titer of the antibody
SDS PAGE - to determine purity of the antibody

Application ELISA.
Western blot (0.1-1 µg/ml).
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin (1/100-1/5,000).
Background Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.
General Readings
  1. Castronovo V et al., Mol Cell Proteomics . Nov;5(11):2083-91.
  2. Li P et al. J Appl Physiol . Jan;102(1):390-8 (2007).
  3. Grigoriadis A et al. Breast Cancer Rest . Oct 2;8(5):R56 (2006).
  4. Utispan K et al. Mol Cancer. 2010;9:13. 
  5. Blumer MJ et al.  J. Anat . Jun;208(6):695-707 (2006). 
  6.  Hosoya A et al. J Histochem Cytochem . Dec;54(12):1371-8 (2006). 
  7.  Gao BB et al. Mol Cell Proteomics. 2008 Dec;7 (12):2399-409. 
  8.  Soltermann A et al. Cancer. 2008 Apr 25;114 (2):124-33.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity Chromatography on Immoblized Recombinant Human OSF2
Buffer System:
0.05 M Phosphate Buffer, 0.1 M NaCl, pH 7.2 without preservatives
State:
Lyophilized (sterile filtered) purified IgG fraction
Azide Free
Reconstitution:
Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Species Reactivity
Species reactivity (tested):
Human, Rat, Mouse, Chicken
Specificity
Specificity:
The antibody recognizes OSF-2.
Gene ID 10631

Accessory Products

Proteins and/or Positive Controls

Proteins for Periostin (3 products)

Catalog No. Species Pres. Purity   Source  

Periostin (transcript variant 2)

Periostin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Periostin (transcript variant 3)

Periostin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Periostin (His-tagged Fusion Protein)

12% SDS-PAGE separation of Human OSF-2: 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa 2. Reduced and heated sample, 5µg/lane 3. Non-reduced and non-heated sample, 5µg/lane. Human Purified > 90 % pure by SDS-PAGE. E. coli
0.1 mg / €470.00
  Acris Antibodies GmbH

Positive controls for Periostin (1 products)

Catalog No. Species Pres. Purity   Source  

POSTN overexpression lysate

POSTN overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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