AP10262PU-N PGCA (1042-1057) antibody

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0.1 mg / €420.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat PGCA

Product Description for PGCA

Rabbit anti Human, Mouse, Rat PGCA.
Properties: (1042-1057)
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for PGCA

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms PGC-A, Particulate Guanylyl Cyclase A
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic C-terminal peptide (aa 1042-1057 for particulate guanylyl cyclase A
AA Sequence:
C-VRT YWL LGE RGC STR G
Property 1042-1057
Control peptide AP10262CP-N
Application Western blot: > 1:500.
Immunoflourescence.
Immunohistochemistry on paraffin sections.
Background Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, the intracellular levels of cGMP ate maintained by the activity of opposing enzymes: synthesizing gualylyl cyclases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate), while they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, sGC contains a heme group and binds NO that activates the enzyme, while particulate GC is stimulated by natriuretic peptides. In response to G-protein couples receptor stimulation, the cGMP can be produced from GTP by either cytoplasmic, soluble guanylate cycl ase (sGC) are heterodimers (a and b polypeptide chains), that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. Particulate GC (PGCs) have 7 different isoforms, PGC-A through PGC-G and are expressed in most tissues in isoform specific manner (See Table 1). There is significant structural homology among various PGCs, there is a large N-terminal extracellular domain (ECD), a single TMD and a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Both PGC-A and PGC-B are phosphorylated at Serine residues in the KLD (2). Non-ionic detergents stimulated particulate guanylate cyclase activity in cerebral cortex of rat 8- to 12-fold while stimulation of soluble enzyme was 1.3- to 2.5-fold. Among various detergents (Duguch et. Al., 2002). It has been shown that a significant number hippocampal astrocytes (67%) contained both soluble and particulate guanylate cyclases in the same cell (2).
Concentration 0.75 - 1.2 mg/ml (lot specific)
General Readings
  1. Wedel B. J and Garbers D. L., Trend Endocrinol. Met. 9, 213-219; 1998
  2. Kobialka M and Gorczyca WA. Acta Biochimica Polonica 47, 517-528, 2000.
  3. Deguch T., Amano E., Nakeane M. J. Neurochem. 27, 1027-1034, 1976.
  4. Teunissen C et. al,. Brain Res. 891, 206-212; 2001.
Storage Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
Stabilization buffer
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, mouse, rat
Specificity
Specificity:
This antibody detects PGCA at aa 1042-1057.

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