discontinued

GTX14784 PGCB antibody

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0.1 mg / €450.00

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Rabbit anti Human, Rat PGCB

GTX14784

Product Description for PGCB

Rabbit anti Human, Rat PGCB.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PGCB

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms PGC-B, Particulate Guanylyl Cyclase B
Presentation Aff - Purified
Reactivity Hu, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic C terminal peptide for particulate guanylyl cyclase B. (unfortunately, the amino acid sequence is considered to be commercially sensitive). All peptides were amidated for conjugation.
Application WB
Background Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, the intracellular levels of cGMP are maintained by the activity of opposing enzymes: synthesizing gualylyl cyclases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate), while they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, soluble GC (sGC) contains a heme group and binds NO that activates the enzyme, while particulate GC (PGCs) are stimulated by natriuretic peptides. Particulate forms of guanylyl cyclases have been shown to function as natriuretic peptide receptors. In response to G-protein coupled receptor stimulation, the cGMP can be produced from GTP by either soluble guanylate cyclase (sGC), or by PGC. The sGC are heterodimers that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. PGCs have 7 different isoforms, PGCA through PGCG and are expressed in most tissues in isoform specific manner. There is significant structural homology among various PGCs, there is a large N-terminal extracellular domain (ECD), a single TMD and a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Guanylyl cyclase B (PGCB) is a receptor for B type brain natriuretic peptide (BNP) and is 78% identical to PGCA in the ECD but only 43% in the CD. The binding of a ligand to the extra-cellular domain of PGCB triggers signal that control central and peripheral cardiovascular homoeostasis. Both PGCA and PGCB are phosphorylated at Serine residues in the KLD. Non-ionic detergents stimulated particulate guanylate cyclase activity in cerebral cortex of rat 8 to 12 fold while stimulation of soluble enzyme was 1.3 to 2.5 fold.
Storage Keep as concentrated solution. Store at 4
Format
Purification:
Immunogen affinity purified
Buffer System:
Preservative: None Constituents: Glycerol, Sodium chloride, HEPES
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
Cross-reacts with Human and Rat. Not yet tested in other species.

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