AP21373BT-N Phospholipase A2 antibody

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1 ml / €340.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Phospholipase A2

AP21373BT-N

Product Description for Phospholipase A2

Rabbit anti Phospholipase A2.
Presentation: Biotin
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Phospholipase A2

Product Category Primary Antibodies
Quantity 1 ml
Synonyms Allergen Api m I, Phosphatidylcholine 2-acylhydrolase
Presentation Biotin
Reactivity bee venom
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P00630
Immunogen:
Phospholipase A2 isolated and purified from bee venom.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
406141
Application This antibody can be used:
In precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
Recommended Dilutions:
non-precipitating antibody-binding assays: 1/1,000-1/40,000.
Background Phospholipase A2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, a precursor of eicosanoids including prostaglandins and leukotrienes.
This enzyme has been proposed to hydrolyze phosphatidylcholine in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.
Phospholipase A2 IIA can exert beneficial action in the context of infectious diseases since recent studies have shown that this enzyme exhibits potent bactericidal effects. Induction of the synthesis of Phospholipase A2 IIA is generally initiated by endotoxin and a limited number of cytokines via paracrine and/or autocrine processes.
Concentration 10 mg/ml
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.
Format
Label:
Biotin
Molar radio:
Biotin/IgG ~6.1
Purification:
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Buffer System:
PBS, pH 7.2 without preservatives.
State:
Lyophilized Hyperimmune IgG fraction.
Reconstitution:
Restore by adding 1 ml of sterile distilled water.
Specificity
Specificity:
Phospholipase A2 from bee venom.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), Block Titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Species:
bee venom.
Gene ID 406141

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