AP21374BT-N Phospholipase A2 antibody

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1 ml / €360.00
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Rabbit anti Phospholipase A2


Product Description for Phospholipase A2

Rabbit anti Phospholipase A2.
Presentation: Biotin
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Phospholipase A2

Product Category Primary Antibodies
Quantity 1 ml
Synonyms Crotoxin basic chain 1, Crotoxin basic chain 2, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 CB1, Phospholipase A2 CB2
Presentation Biotin
Reactivity Crotalus durissus terrificus
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Phospholipase A2 isolated and purified from Crotalus durissus terrificus venom. Freund’s adjuvant is used in the first step of the immunization procedure.
Application Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
Recommended working dilutions: vary widely, but may be between 1/1000 and 1/20000.
Concentration 10,0 mg/ml
Storage Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Marker: N-Hydroxysuccinimidobiotin.Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps.After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life
Molar radio:
Biotin/IgG ~4.7
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
Lyophilized hyperimmune IgG fraction
Restore by adding 1.0 ml of sterile distilled water
Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase A2 from Crotalus durissus terrificus venom.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

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