AM20046PU-N Phosphotryrosine antibody

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0.1 mg / €370.00
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Quick Overview

Mouse anti Phosphotryrosine G104


Product Description for Phosphotryrosine

Mouse anti Phosphotryrosine G104.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Phosphotryrosine

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications C, ICC/IF, IP, WB
Clonality Monoclonal
Clone G104
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of Keyhole Limpet Hemocyanin with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide.
Application Western blot: 1/1000 (1,2).
1 μg/ml of this antibody was sufficient for detection of phosphorylated tyrosine residues in 10 μg of Rat tissue lysate by colorimetric Immunoblot analysis using Goat anti-Rat IgG-HRP as the secondary antibody.
Immunoprecipitation (9).
Immunofluorescence (2).
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3).
Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).
Concentration 1.0 mg/ml
General Readings
  1. Garton A.J., Flint A.J., and Tonks N.K. (1996) Mol and Cell Bio 16(11): 6408-6418.
  2. Garton A.J., and Tonks N.K. (1999) J Bio Chem. 274(6): 3811-3818.
  3. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.
  4. Blume-Jensen P. and Hunter T. (2001) Nature 411:355- 365.
  5. Downward J. (2001) Nature 411: 759-762.
  6. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.
  7. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352.
  8. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656.
  9. Tiganis T., Kemp B.E., and Tonks N.K. (1999) J. Bio Chem. 274(39): 27768-27775.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G Chromatography.
Buffer System:
PBS, pH 7.4 containing 50% Glycerol and 0.09% Sodium Azide as preservative.
Liquid purified IgG fraction
Reacts with Phosphotyrosine and detects the presence of Phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.
Does not cross react with Phosphoserine or Phosphothreonine.

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