NB110-96881 Phosphotyrosine (13F9) antibody

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Mouse anti Phosphotyrosine (13F9) 13F9


Product Description for Phosphotyrosine (13F9)

Mouse anti Phosphotyrosine (13F9) 13F9.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for Phosphotyrosine (13F9)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications E, F, IP, P, WB
Clonality Monoclonal
Clone 13F9
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Phosphotyrosine conjugated to KLH
Isotype control AM03095PU-N
Application ELISA, Western Blot, ELISA and Western Blot ELISA results tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1ug of conjugate. The starting dilution of antibody was 1:1000 and each point on the x-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) and TMB substrate was used for detection., ELISA 1:2000 to 1:10000, Western Blot 1:2000 to 1:10000
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
IgG purified
Buffer System:
0.02% Sodium Azide
IgG purified
Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Does not cross react with phosphoserine or phosphothreonine.

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