AM33265PU-T Phosphotyrosine antibody

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20 µg / €160.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti All Species Phosphotyrosine SPM102

Product Description for Phosphotyrosine

Mouse anti All Species Phosphotyrosine SPM102.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Phosphotyrosine

Product Category Primary Antibodies
Quantity 20 µg
Presentation Purified
Reactivity All Species
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone SPM102
Host Mouse
Isotype IgG2b
Molecular weight Depends upon the phosphorylated target
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Phosphotyrosine conjugated to KLH
Application ELISA: Use BSA free Antibody for Coating.
Western Blot: 0.5-1 µg/ml.
Flow Cytometry: 0.5-1 µg/106 cells.  
Immunofluorescence: 1-2 µg/ml. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed 
Paraffin Sections: 1-2 µg/ml for 30 minutes at RT. 
No special pretreatment is required for staining of formalin/paraffin tissues. 
Recommended Positive Control: MCF-7, MDA-231, T47-D cells or breast carcinoma.
Background Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes.  Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases.  The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates.  Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation.
Concentration 0.2 mg/ml
General Readings
  1. Glenney JR, Zokas L, Kamps MP. Monoclonal antibodies to phosphotyrosine. J Immunol Methods. 1988 May 9;109(2):277-85. PubMed PMID: 2452204.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins.
This Monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Cellular LocalizationDepends upon the location of phosphorylated target.

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