BP2240 Pichia pastoris antibody

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Rabbit anti Pichia pastoris


Product Description for Pichia pastoris

Rabbit anti Pichia pastoris.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Pichia pastoris

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Whole intact cells of P. pastoris.
Application Western blot: > 1/100.
Immunofluorescence assay: 1/800. This product may also be used in place of neat antiserum in almost any appropriate antibody-based technique.
Also suitable for conjugation purposes. Antibody is useful for detection/removal of contaminants from recombinant preps.
Suitability for use in ELISA and Immunohistochemistry has not been determined but use in such assays should not necessarily be excluded.
Background Pichia pastoris, a methylotrophic yeast, has been used as a heterologous protein expression system. It combines many of the benefits of E. coli expression with the advantages of expression in an eukaryotic system such as protein processing, folding, and posttranslational modifications.
Pichia pastoris is capable of metabolising methanol as the sole carbon source. The first step in the metabolism of methanol is the oxidation of methanol to formaldehyde by the enzyme, alcohol oxidase. Expression of this enzyme, coded for by the AOX1 gene, is tightly regulated and induced by methanol to very high levels:- typically > 30% of the total soluble protein in the cells.
Concentration 4-5 mg/ml (OD280 nm, E0.1% = 1.4)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A Chromatography
Buffer System:
0.01M PBS, pH 7.2
0.09% Sodium Azide
Liquid purified Ig fraction (> 95% pure)
Reacts with all antigens.
Cross-reactive with Candida albicans (strong) and S. cerevisiae (weak).

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