NB100-65067 Pichia pastoris antibody

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Rabbit anti Yeast Pichia pastoris


Product Description for Pichia pastoris

Rabbit anti Yeast Pichia pastoris.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Pichia pastoris

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Aff - Purified
Reactivity Ye
Applications C, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

P. pastoris whole cells
Application immunofluorescence, Immunohistochemistry-Frozen, Western Blot, Immunohistochemistry-Frozen, Western Blot and Immunofluorescence, Immunohistochemistry-Frozen immunofluorescence Western Blot
Background Pichia pastoris, a methylotrophic yeast, has been used as a heterologous protein expression system. It combines many of the benefits of E. coli expression with the advantages of expression in an eukaryotic system such as protein processing, folding, and posttranslational modifications. Pichia pastoris is capable of metabolizing methanol as the sole carbon source. The first step in the metabolism of methanol is the oxidation of methanol to formaldehyde by the enzyme, alcohol oxidase. Expression of this enzyme, coded for by the AOX1 gene, is tightly regulated and induced by methanol to very high levels: typically >30% of the total soluble protein in the cells.
Concentration 5 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
IgG purified
Buffer System:
0.09% Sodium Azide
IgG purified
Aff - Purified
Reacts with all Pichia pastoris antigens and displays cross reactivity with C. albicans and to a lesser extent S. cerevisiae. This is useful for detection/removal of contaminants from recombinant preparations.

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