AP31457FC-N Pigeon IgG (H+L chain) antibody

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2 ml / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Pigeon Pigeon IgG

AP31457FC-N

Product Description for Pigeon IgG

Rabbit anti Pigeon Pigeon IgG.
Properties: (H+L chain)
Presentation: FITC
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Pigeon IgG

Product Category Primary Antibodies
Quantity 2 ml
Synonyms Pigeon Immunoglobulin G
Presentation FITC
Reactivity Pigeon
Applications C, Dot, E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified normal IgG isolated from pooled pigeon serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Property H+L chain
Application Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of pigeon origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Concentration 10 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein isothiocyanate isomer 1
FITC
Absorption emission:
492nm / 515nm
Molar radio:
1,8
Purification:
DEAE-column Chromatography
Buffer System:
PBS, pH 7.2
No preservative added - No foreign proteins added.
State:
Lyophilised hyperimmune Ig fraction
Reconstitution:
Restore with 2 ml sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in pigeon serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the concentration of the reactants and the sensitivity of the assay format. Cross-reactivity of this antiserum has not been tested in detail, however in immunoelectrophoresis a reaction with chicken, duck and turkey has been observed.
Species:
Pigeon.

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