AP09228PU-N Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

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0.1 mg / €330.00
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Rabbit anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Product Description for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Rabbit anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST).
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide
AA Sequence:
1 mdptdlsfsp deinklietg lntveyftsq qvtgtsslgk ntippgvtgl ltnaaeakiq
61 estnhqkgsv gggakpkkpr pkiaivpadd ktvpgkpipn pllgldstps tqtvldlsgk
121 tlpsgsykgv klakfgkenl mtrfieepre npiatsspid fkrgrdtggf hrreysigwv
181 gdevkvtewc npscspitaa arrfectchq cpvtcsecer dt
V protein [Simian parainfluenza virus 5], 222 aa, predicted MW 167.7 kDa
Application ELISA: 1:10,000 - 1:60,000.
Western blot: 1:5,000 - 1:10,000.
Immunohistochemistry: 1:500 - 1:3,000.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Liquid sterile filtered Ig fraction
Aff - Purified
This affinity-purified antibody is directed against V5 motif and is useful in determining its presence in various assays. This polyclonal anti-V5-tag antibody detects over-expressed proteins containing the V5 epitope tag. To date this antibody has reacted with all V5 tagged proteins tested so far.
The antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to either the carboxy- terminal end of targeted proteins in transfected or transformed cells. Although not yet tested, expect reactivity with recombinant proteins prepared with the V5-epitope tag fused to the amino terminal end as well.

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