NBP1-21645 Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

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0.1 mg / €360.00
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Quick Overview

Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-Pk2

Product Description for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-Pk2.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications E, IP, WB
Clonality Monoclonal
Clone SV5-Pk2
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.
Isotype control AM03095PU-N
Application ELISA, immunoprecipitation, Western Blot, ELISA, Immunoprecipitation and Western Blot, ELISA , immunoprecipitation , Western Blot 1:100 - 1:500
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
Concentration 1 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
protein G purified
Buffer System:
0.09% Sodium Azide
Purity:
protein G purified
State:
Purified
Specificity
Specificity:
NBP1-21645 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. NBP1-21645 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
Species:
Virus

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