SM1691B Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

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0.1 mg / €510.00
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Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-PK1


Product Description for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-PK1.
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Biotin
Applications C, E, WB
Clonality Monoclonal
Clone SV5-PK1
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Add. information This product is manufactured under an exclusive license from the University of St. Andrews,UK.
Application ELISA.
Western Blot: 1/1000-1/5000.
Immunohistochemistry on Frozen Sections.
Concentration 1.0 mg/ml
General Readings
  1. Southern JA, Young DF, Heaney F, Baumgärtner WK, Randall RE. Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J Gen Virol. 1991 Jul;72 ( Pt 7):1551-7. PubMed PMID: 1713260.
  2. Hanke T, Szawlowski P, Randall RE. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J Gen Virol. 1992 Mar;73 ( Pt 3):653-60. PubMed PMID: 1372038.
  3. Randall RE, Hanke T, Young D, Southern JA. Two-tag purification of recombinant proteins for the construction of solid matrix-antibody-antigen (SMAA) complexes as vaccines. Vaccine. 1993 Sep;11(12):1247-52. PubMed PMID: 7504859.
  4. Sanchez Garcia J, Ciufo LF, Yang X, Kearsey SE, MacNeill SA. The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit. Nucleic Acids Res. 2004 Jun 1;32(10):3005-16. Print 2004. PubMed PMID: 15173383. (Free PMC Article available, 6 images available)
  5. Randall RE, Young D, Hanke T, Szawlowski P, Botting C. Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming. Vaccine. 1994 Mar;12(4):351-8. PubMed PMID: 8178558.
  6. Hanke, T. et al. (1995) Attachment of epitope to C-terminus of recombinant SIV gp160 facilitates purification while preserving CD4 binding. J. Virol. Methods 149-156.
  7. Jaffray, E. et al. (1995) Domain structure of IkBa and sites of interaction with NF-kB p65. Mol. Cell. Biol. 15: 2166-2172.
  8. Rodriguez MS, Michalopoulos I, Arenzana-Seisdedos F, Hay RT. Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol Cell Biol. 1995 May;15(5):2413-9. PubMed PMID: 7739525. (Free PMC Article available)
  9. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696.
  10. Hirst, K. et al. (1994) The transcription factor, CDK, its cyclin and their regulator; directing the transcription response to a nutritional signal. EMBO J. 13: 5410-5420.
  11. Dunn C, O'Dowd A, Randall RE. Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J Immunol Methods. 1999 Apr 22;224(1-2):141-50. PubMed PMID: 10357214.
  12. Young DF, Chatziandreou N, He B, Goodbourn S, Lamb RA, Randall RE. Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells. J Virol. 2001 Apr;75(7):3363-70. PubMed PMID: 11238862. (Free PMC Article available, 6 images available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium Azide as preservative
Liquid purified IgG fraction
This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (the 9 amino acid epitope is underlined)

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