SM1691D5 Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

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0.5 mg / €530.00

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Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-Pk1


Product Description for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-Pk1.
Presentation: DyLight549

Properties for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms Pk-Tag, V5-Tag
Presentation DyLight549
Clonality Monoclonal
Clone SV5-Pk1
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Application Immunofluorescence (1/100-1/500).
Membrane permeabilisation with 0.5% IGEPAL is required.
Concentration 1.0 mg/ml
General Readings
  1. Southern JA, Young DF, Heaney F, Baumgärtner WK, Randall RE. Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J Gen Virol. 1991 Jul;72 ( Pt 7):1551-7. PubMed PMID: 1713260.
  2. Hanke T, Szawlowski P, Randall RE. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J Gen Virol. 1992 Mar;73 ( Pt 3):653-60. PubMed PMID: 1372038.
  3. Randall RE, Hanke T, Young D, Southern JA. Two-tag purification of recombinant proteins for the construction of solid matrix-antibody-antigen (SMAA) complexes as vaccines. Vaccine. 1993 Sep;11(12):1247-52. PubMed PMID: 7504859.
  4. Randall RE, Young D, Hanke T, Szawlowski P, Botting C. Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming. Vaccine. 1994 Mar;12(4):351-8. PubMed PMID: 8178558.
  5. Hanke T, Young DF, Doyle C, Jones I, Randall RE. Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding. J Virol Methods. 1995 May;53(1):149-56. PubMed PMID: 7543487.
  6. Jaffray, E. et al. (1995) Domain structure of IkBa and sites of interaction with NF-kB p65. Mol. Cell. Biol. 15: 2166-2172.
  7. Rodriguez MS, Michalopoulos I, Arenzana-Seisdedos F, Hay RT. Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol Cell Biol. 1995 May;15(5):2413-9. PubMed PMID: 7739525. (Free PMC Article available)
  8. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696.
  9. Hirst, K. et al. (1994) The transcription factor, CDK, its cyclin and their regulator; directing the transcription response to a nutritional signal. EMBO J. 13: 5410-5420.
  10. Dunn C, O'Dowd A, Randall RE. Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J Immunol Methods. 1999 Apr 22;224(1-2):141-50. PubMed PMID: 10357214.
  11. Young DF, Chatziandreou N, He B, Goodbourn S, Lamb RA, Randall RE. Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells. J Virol. 2001 Apr;75(7):3363-70. PubMed PMID: 11238862. (Free PMC Article available, 6 images available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Liquid purified IgG fraction containing 0.09% Sodium Azide as preservative.
This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used.
The 14 amino acid epitope is: gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).

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