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SM1691PS Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

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Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-PK1

SM1691PS

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  • SM1691PS

Product Description for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-PK1.
Presentation: Purified
Product is tested for Flow Cytometry, Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications C, E, F, ICC/IF, IP, R, WB
Clonality Monoclonal
Clone SV5-PK1
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Paramyxovirus Simian-Virus 5 (SV5)
Remarks:
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Isotype control AM03096PU-N
Application ELISA: 1/1000-1/5000.
Western Blot: 1/1000-1/5000.
Immunoprecipitation.
Radioimmunoassays.
Immunofluorescence.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Concentration 1.0 mg/ml
General Readings 1. Southern JA, Young DF, Heaney F, Baumgärtner WK, Randall RE. Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J Gen Virol. 1991 Jul;72 ( Pt 7):1551-7. PubMed PMID: 1713260.
2. Hanke T, Szawlowski P, Randall RE. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J Gen Virol. 1992 Mar;73 ( Pt 3):653-60. PubMed PMID: 1372038.
3. Randall RE, Hanke T, Young D, Southern JA. Two-tag purification of recombinant proteins for the construction of solid matrix-antibody-antigen (SMAA) complexes as vaccines. Vaccine. 1993 Sep;11(12):1247-52. PubMed PMID: 7504859.
4. Randall RE, Young D, Hanke T, Szawlowski P, Botting C. Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming. Vaccine. 1994 Mar;12(4):351-8. PubMed PMID: 8178558.
5. Hanke T, Young DF, Doyle C, Jones I, Randall RE. Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding. J Virol Methods. 1995 May;53(1):149-56. PubMed PMID: 7543487.
6. Jaffray E, Wood KM, Hay RT. Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65. Mol Cell Biol. 1995 Apr;15(4):2166-72. PubMed PMID: 7891711.
7. Rodriguez MS, Michalopoulos I, Arenzana-Seisdedos F, Hay RT. Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol Cell Biol. 1995 May;15(5):2413-9. PubMed PMID: 7739525.
8. Arenzana-Seisdedos F, Thompson J, Rodriguez MS, Bachelerie F, Thomas D, Hay RT. Inducible nuclear expression of newly synthesized I kappa B alpha negatively regulates DNA-binding and transcriptional activities of NF-kappa B. Mol Cell Biol. 1995 May;15(5):2689-96. PubMed PMID: 7739549.
9. Hirst K, Fisher F, McAndrew PC, Goding CR. The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal. EMBO J. 1994 Nov 15;13(22):5410-20. PubMed PMID: 7957107.
10. Dunn C, O'Dowd A, Randall RE. Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J Immunol Methods. 1999 Apr 22;224(1-2):141-50. PubMed PMID: 10357214.
11. Young DF, Chatziandreou N, He B, Goodbourn S, Lamb RA, Randall RE. Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells. J Virol. 2001 Apr;75(7):3363-70. PubMed PMID: 11238862.
12. Sanchez Garcia J, Ciufo LF, Yang X, Kearsey SE, MacNeill SA. The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit. Nucleic Acids Res. 2004 Jun 1;32(10):3005-16. Print 2004. PubMed PMID: 15173383.
13. Liebau MC, Höpker K, Müller RU, Schmedding I, Zank S, Schairer B, et al. Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of nephrocystin-1 to control targeting to monocilia. J Biol Chem. 2011 Apr 22;286(16):14237-45. doi: 10.1074/jbc.M110.165464. Epub 2011 Feb 28. PubMed PMID: 21357692.
14. Liebau, M.C. et al. (2011) Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of Nephrocystin-1 to control targeting to monocilia. J Biol Chem. Feb 28. [Epub ahead of print]
15. Björk JK, Sandqvist A, Elsing AN, Kotaja N, Sistonen L. miR-18, a member of Oncomir-1, targets heat shock transcription factor 2 in spermatogenesis. Development. 2010 Oct;137(19):3177-84. doi: 10.1242/dev.050955. Epub 2010 Aug 19. PubMed PMID: 20724452.
16. Boggio R, Passafaro A, Chiocca S. Targeting SUMO E1 to ubiquitin ligases: a viral strategy to counteract sumoylation. J Biol Chem. 2007 May 25;282(21):15376-82. Epub 2007 Mar 28. PubMed PMID: 17392274.
17. Gallazzini M, Heussler GE, Kunin M, Izumi Y, Burg MB, Ferraris JD. High NaCl-induced activation of CDK5 increases phosphorylation of the osmoprotective transcription factor TonEBP/OREBP at threonine 135, which contributes to its rapid nuclear localization. Mol Biol Cell. 2011 Mar 1;22(5):703-14. doi: 10.1091/mbc.E10-08-0681. Epub 2011 Jan 5. PubMed PMID: 21209322.
18. Hadler KS, Huber T, Cassady AI, Weber J, Robinson J, Burrows A, et al. Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases? BMC Res Notes. 2008 Sep 4;1:78. doi: 10.1186/1756-0500-1-78. PubMed PMID: 18771593.
19. Zhao A, Nunez-Cruz S, Li C, Coukos G, Siegel DL, Scholler N. Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform. J Immunol Methods. 2011 Jan 5;363(2):221-32. doi: 10.1016/j.jim.2010.09.001. Epub 2010 Sep 15. PubMed PMID: 20837020.
20. Patino GA, Brackenbury WJ, Bao Y, Lopez-Santiago LF, O'Malley HA, Chen C, et al. Voltage-gated Na+ channel β1B: a secreted cell adhesion molecule involved in human epilepsy. J Neurosci. 2011 Oct 12;31(41):14577-91. doi: 10.1523/JNEUROSCI.0361-11.2011. PubMed PMID: 21994374.
21. Gatherer, D. et al. (2011) High-resolution human cytomegalovirus transcriptome. Proc Natl Acad Sci U S A. Nov 22. [Epub ahead of print].
22. Mahuzier, A. et al. (2012) Dishevelled stabilization by the ciliopathy protein Rpgrip1l is essential for planar cell polarity. J Cell Biol. Aug 27. [Epub ahead of print].
23. Zhao C, Denison C, Huibregtse JM, Gygi S, Krug RM. Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways. Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10200-5. Epub 2005 Jul 11. PubMed PMID: 16009940.
24. Singh A, Minia I, Droll D, Fadda A, Clayton C, Erben E. Trypanosome MKT1 and the RNA-binding protein ZC3H11: interactions and potential roles in post-transcriptional regulatory networks. Nucleic Acids Res. 2014 Apr;42(7):4652-68. doi: 10.1093/nar/gkt1416. Epub 2014 Jan 26. PubMed PMID: 24470144.
25. Mui MZ et al. (2015) The human adenovirus type 5 E4orf4 protein targets two phosphatase regulators of the Hippo signaling pathway. J Virol. pii: JVI.03710-14.
26. Shi X et al. (2016) Bunyamwera orthobunyavirus glycoprotein precursor is processed by
cellular signal peptidase and signal peptide peptidase. Proc Natl Acad Sci U S A. 113 (31):8825-30.
27. Ng, M.Y. et al. (2017) Activation of MAPK/ERK signaling by Burkholderia pseudomallei cycle inhibiting factor (Cif). PLoS One. 12 (2): e0171464.
28. Voskarides, K. et al. (2017) A functional variant in NEPH3 gene confers high risk of renal failure in primary hematuric glomerulopathies. Evidence for predisposition to microalbuminuria in the general population. PLoS One. 12 (3): e0174274.
29. Malik, S. et al. (2015) Adrenocorticotropic Hormone (ACTH) Responses Require Actions of the Melanocortin-2 Receptor Accessory Protein on the Extracellular Surface of the Plasma Membrane. J Biol Chem. 290 (46): 27972-85.
30. Carrocci, T.J. et al. (2017) SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. Nucleic Acids Res. 45 (8): 4837-4852.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction from Tissue Culture Supernatant
Purified
Specificity
Specificity:
Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V
proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect
recombinant proteins, some of which include transmembrane and secreted proteins, that have
labelled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).

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