SM1691PS Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

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0.1 mg / €250.00
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Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-PK1

SM1691PS

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  • SM1691PS

Product Description for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Mouse anti Pk (V5) Epitope Tag (GKPIPNPLLGLDST) SV5-PK1.
Presentation: Purified
Product is tested for Flow Cytometry, Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications C, E, F, ICC/IF, IP, R, WB
Clonality Monoclonal
Clone SV5-PK1
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mice were infected with the Paramyxovirus SV5, Simian-Virus 5.
Remarks:
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Isotype control AM03096PU-N
Application ELISA: 1/1000-1/5000.
Western Blot: 1/1000-1/5000.
Immunoprecipitation.
Radioimmunoassays.
Immunofluorescence.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Concentration 1.0 mg/ml
General Readings
  1. Southern JA, Young DF, Heaney F, Baumgärtner WK, Randall RE. Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J Gen Virol. 1991 Jul;72 ( Pt 7):1551-7. PubMed PMID: 1713260.
  2. Hanke T, Szawlowski P, Randall RE. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J Gen Virol. 1992 Mar;73 ( Pt 3):653-60. PubMed PMID: 1372038.
  3. Randall RE, Hanke T, Young D, Southern JA. Two-tag purification of recombinant proteins for the construction of solid matrix-antibody-antigen (SMAA) complexes as vaccines. Vaccine. 1993 Sep;11(12):1247-52. PubMed PMID: 7504859.
  4. Randall RE, Young D, Hanke T, Szawlowski P, Botting C. Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming. Vaccine. 1994 Mar;12(4):351-8. PubMed PMID: 8178558.
  5. Hanke T, Young DF, Doyle C, Jones I, Randall RE. Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding. J Virol Methods. 1995 May;53(1):149-56. PubMed PMID: 7543487.
  6. Jaffray E, Wood KM, Hay RT. Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65. Mol Cell Biol. 1995 Apr;15(4):2166-72. PubMed PMID: 7891711. (Free PMC Article available)
  7. Rodriguez MS, Michalopoulos I, Arenzana-Seisdedos F, Hay RT. Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol Cell Biol. 1995 May;15(5):2413-9. PubMed PMID: 7739525. (Free PMC Article available)
  8. Arenzana-Seisdedos F, Thompson J, Rodriguez MS, Bachelerie F, Thomas D, Hay RT. Inducible nuclear expression of newly synthesized I kappa B alpha negatively regulates DNA-binding and transcriptional activities of NF-kappa B. Mol Cell Biol. 1995 May;15(5):2689-96. PubMed PMID: 7739549. (Free PMC Article available)
  9. Hirst K, Fisher F, McAndrew PC, Goding CR. The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal. EMBO J. 1994 Nov 15;13(22):5410-20. PubMed PMID: 7957107. (Free PMC Article available)
  10. Dunn C, O'Dowd A, Randall RE. Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J Immunol Methods. 1999 Apr 22;224(1-2):141-50. PubMed PMID: 10357214.
  11. Young DF, Chatziandreou N, He B, Goodbourn S, Lamb RA, Randall RE. Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells. J Virol. 2001 Apr;75(7):3363-70. PubMed PMID: 11238862. (Free PMC Article available, 6 images available)
  12. Sanchez Garcia J, Ciufo LF, Yang X, Kearsey SE, MacNeill SA. The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit. Nucleic Acids Res. 2004 Jun 1;32(10):3005-16. Print 2004. PubMed PMID: 15173383. (Free PMC Article available, 6 images available)
  13. Liebau MC, Höpker K, Müller RU, Schmedding I, Zank S, Schairer B, et al. Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of nephrocystin-1 to control targeting to monocilia. J Biol Chem. 2011 Apr 22;286(16):14237-45. doi: 10.1074/jbc.M110.165464. Epub 2011 Feb 28. PubMed PMID: 21357692. (Free PMC Article available, 5 images available)
  14. Liebau, M.C. et al. (2011) Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of Nephrocystin-1 to control targeting to monocilia. J Biol Chem. Feb 28. [Epub ahead of print]
  15. Björk JK, Sandqvist A, Elsing AN, Kotaja N, Sistonen L. miR-18, a member of Oncomir-1, targets heat shock transcription factor 2 in spermatogenesis. Development. 2010 Oct;137(19):3177-84. doi: 10.1242/dev.050955. Epub 2010 Aug 19. PubMed PMID: 20724452.
  16. Boggio R, Passafaro A, Chiocca S. Targeting SUMO E1 to ubiquitin ligases: a viral strategy to counteract sumoylation. J Biol Chem. 2007 May 25;282(21):15376-82. Epub 2007 Mar 28. PubMed PMID: 17392274.
  17. Gallazzini M, Heussler GE, Kunin M, Izumi Y, Burg MB, Ferraris JD. High NaCl-induced activation of CDK5 increases phosphorylation of the osmoprotective transcription factor TonEBP/OREBP at threonine 135, which contributes to its rapid nuclear localization. Mol Biol Cell. 2011 Mar 1;22(5):703-14. doi: 10.1091/mbc.E10-08-0681. Epub 2011 Jan 5. PubMed PMID: 21209322. (Free PMC Article available, 8 images available)
  18. Hadler KS, Huber T, Cassady AI, Weber J, Robinson J, Burrows A, et al. Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases? BMC Res Notes. 2008 Sep 4;1:78. doi: 10.1186/1756-0500-1-78. PubMed PMID: 18771593. (Free PMC Article available, 5 images available)
  19. Zhao A, Nunez-Cruz S, Li C, Coukos G, Siegel DL, Scholler N. Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform. J Immunol Methods. 2011 Jan 5;363(2):221-32. doi: 10.1016/j.jim.2010.09.001. Epub 2010 Sep 15. PubMed PMID: 20837020. (Free PMC Article available, 6 images available)
  20. Patino GA, Brackenbury WJ, Bao Y, Lopez-Santiago LF, O'Malley HA, Chen C, et al. Voltage-gated Na+ channel β1B: a secreted cell adhesion molecule involved in human epilepsy. J Neurosci. 2011 Oct 12;31(41):14577-91. doi: 10.1523/JNEUROSCI.0361-11.2011. PubMed PMID: 21994374. (Free PMC Article available, 11 images available)
  21. Gatherer, D. et al. (2011) High-resolution human cytomegalovirus transcriptome. Proc Natl Acad Sci U S A. Nov 22. [Epub ahead of print].
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  24. Singh A, Minia I, Droll D, Fadda A, Clayton C, Erben E. Trypanosome MKT1 and the RNA-binding protein ZC3H11: interactions and potential roles in post-transcriptional regulatory networks. Nucleic Acids Res. 2014 Apr;42(7):4652-68. doi: 10.1093/nar/gkt1416. Epub 2014 Jan 26. PubMed PMID: 24470144. (Free PMC Article available, 8 images available)
  25. Mui MZ et al. (2015) The human adenovirus type 5 E4orf4 protein targets two phosphatase regulators of the Hippo signaling pathway. J Virol. pii: JVI.03710-14.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction from Tissue Culture Supernatant
Purified
Specificity
Specificity:
This SV5-Pk1 antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr (The 9 amino acid epitope is underlined).

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