NB120-5815 PKA alpha + beta antibody

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Rabbit anti Human, Mouse PKA alpha + beta


Product Description for PKA alpha + beta

Rabbit anti Human, Mouse PKA alpha + beta.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PKA alpha + beta

Product Category Primary Antibodies
Quantity 0.05 ml
Presentation Aff - Purified
Reactivity Hu, Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic phosphopeptide derived from a region of human PKA that contains threonine 197.
Background c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP. The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition. The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop. Phosphorylation of threonine 197 is critical for PKA maturation, optimal conformation and catalytic activity and plays a key role in mediating its biological functions.
General Readings General / background references:Winklhofer M et al. Analysis of phosphorylation-dependent modulation of Kv1.1 potassium channels. Neuropharmacology 44:829-42 (2003). PubMed PMID: 12681381
Aimes RT et al. Serine-53 at the tip of the glycine-rich loop of cAMP-dependent protein kinase: role in catalysis, P-site specificity, and interaction with inhibitors. Biochemistry 39:8325-32 (2000). PubMed PMID: 10889042
Taylor JR et al. Activation of cAMP-dependent protein kinase A in prefrontal cortex impairs working memory performance. J Neurosci 19:RC23 (1999). PubMed PMID: 10479716
Yonemoto W et al. Autophosphorylation of the catalytic subunit of cAMP-dependent protein kinase in Escherichia coli. Protein Eng 10:915-25 (1997). PubMed PMID: 9415441
Immunogen affinity purified
Buffer System:
Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA. pH 7.3
Immunogen affinity purified
Aff - Purified
Cross-reacts with Human and Mouse.Expected to cross-react with Pig (100% identity with immunogen), Cow (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species.

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