discontinued

NSB988 PKA alpha + beta antibody

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0.1 ml / €360.00

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Rabbit anti Human, Mouse PKA alpha + beta

Product Description for PKA alpha + beta

Rabbit anti Human, Mouse PKA alpha + beta.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PKA alpha + beta

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu, Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic phosphopeptide derived from a region of human PKA that contains threonine 197.
Application Western Blot, WB: Detects a band of approximately 42 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., Western Blot 0.1 - 0.75 ug/ml
Background c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP. The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition. The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop. Phosphorylation of threonine 197 is critical for PKA maturation, optimal conformation and catalytic activity and plays a key role in mediating its biological functions.
General Readings General / background references:Winklhofer M et al. Analysis of phosphorylation-dependent modulation of Kv1.1 potassium channels. Neuropharmacology 44:829-42 (2003). PubMed PMID: 12681381
Aimes RT et al. Serine-53 at the tip of the glycine-rich loop of cAMP-dependent protein kinase: role in catalysis, P-site specificity, and interaction with inhibitors. Biochemistry 39:8325-32 (2000). PubMed PMID: 10889042
Taylor JR et al. Activation of cAMP-dependent protein kinase A in prefrontal cortex impairs working memory performance. J Neurosci 19:RC23 (1999). PubMed PMID: 10479716
Yonemoto W et al. Autophosphorylation of the catalytic subunit of cAMP-dependent protein kinase in Escherichia coli. Protein Eng 10:915-25 (1997). PubMed PMID: 9415441
5. Moore, M.J., et al. Phosphorylation of the catalytic subunit of protein kinase A. autophosphorylation versus phosphorylation by phosphoinositide-dependent kinase-1. J. Biol. Chem. 277(49): 47878-47884, 2002.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
This exibited a preference for PKA catalytic subunit beta in some tested cell lines.
Species:
Human, Mouse

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