discontinued

NSB963 PKC beta 1 antibody

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0.1 ml / €360.00

Quick Overview

Rabbit anti Human PKC beta 1

Product Description for PKC beta 1

Rabbit anti Human PKC beta 1.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PKC beta 1

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic phosphopeptide derived from a region of human PKC beta 1 that contains threonine 642.
Add. information Research Areas: Cancer
Application Western Blot, Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., Western Blot 1/1000
Background Protein Kinase C beta 1 (PKC beta 1) is an 80 kDa member of the conventional group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. PKC beta 1 is phosphorylated on three sites, threonine 500 in the activation loop, threonine 642 in the turn loop and serine 661 in the hydrophobic loop. Autophosphorylation of PKC beta 1 on threonine 642 is crucial for its activation and catalytic activity. Threonine 642 mediates PKC beta 1 ability to activate c-Fos in response to phorbol esters.
General Readings General / background references:Gao T & Newton AC The turn motif is a phosphorylation switch that regulates the binding of Hsp70 to protein kinase C. J Biol Chem 277:31585-92 (2002). PubMed PMID: 12080070
Edwards AS et al. Carboxyl-terminal phosphorylation regulates the function and subcellular localization of protein kinase C betaII. J Biol Chem 274:6461-8 (1999). PubMed PMID: 10037738
Keranen LM et al. Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. Curr Biol 5:1394-1403 (1995). PubMed PMID: 8749392
Newton AC Protein kinase C: structure, function, and regulation. J Biol Chem 270:28495-8 (1995). PubMed PMID: 7499357
5. Paula, D., et al. Oxidative stress induces increase intracellular amyloid beta-protein production and selective activation of betaI and betaII PKCs in NT2 cells. Biochem. Biophys. Res. Comm. 268(2):642-646, 2000.
6. Zhang, J. et al. Phophorylation of Thr642 is an early event in the processing of newly synthesized protein kinase C beta1 and is essential for its activation. J. Biol. Chem. 269(30): 19578-19584, 1994.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
This does not react with PKC beta 2 [pT641], alpha [pT638], nu [pT655], epsilon [pT710], iota [pT555], eta [pT655], or zeta [pT560] as determined by peptide competition experiments.
Species:
Human

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