NSB955 PKC beta 1 + PKC beta 2 antibody

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Rabbit anti Human PKC beta 1 + PKC beta 2

Product Description for PKC beta 1 + PKC beta 2

Rabbit anti Human PKC beta 1 + PKC beta 2.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PKC beta 1 + PKC beta 2

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic phosphopeptide derived from a region of human PKC beta 1 & 2 that contains threonine 500.
Add. information Research Areas: Cancer
Application Western Blot, WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., Western Blot
Background Protein Kinase C beta (PKC beta) is an 80 kDa member of the conventional group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. PKC beta has been implicated in diabetes and carcinogenesis. PKC beta isoforms 1 & 2 are phosphorylated on three sites, threonine 500 in the activation loop, beta 1 threonine 642 (beta 2 641) in the turn loop and beta 1 serine 661 (beta 2 660) in the hydrophobic loop. Phosphorylation of PKC beta 1 & 2 on threonine 500 by PDK1 is a prerequisite for its autophosphoylation and catalytic competence.
General Readings General / background references:Newton AC Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigm. Biochem J 370:361-71 (2003). PubMed PMID: 12495431
Yoshida M et al. Involvement of PKC betaII in anti-proliferating action of a new antitumor compound gnidimacrin. Int J Cancer 105:601-6 (2003). PubMed PMID: 12740906
Keranen LM et al. Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. Curr Biol 5:1394-1403 (1995). PubMed PMID: 8749392
Newton AC Protein kinase C: structure, function, and regulation. J Biol Chem 270:28495-8 (1995). PubMed PMID: 7499357
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
Aff - Purified
This cross-reacts with PKC alpha [pT497] (88% homologous) and partially cross-reacts with PKC gamma [pT514] (63% homologous) and epsilon [pT566] (75% homologous), as determined by peptide competition experiments.

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