NSB956 PKC gamma antibody

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0.1 ml / €360.00

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Rabbit anti Human PKC gamma

Product Description for PKC gamma

Rabbit anti Human PKC gamma.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PKC gamma

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic phosphopeptide derived from a region of human PKC gamma that contains threonine 514.
Application Western Blot, Western Blot Detects a band of approximately 80 kDa. , Western Blot 0.5 - 1.0 ug/ml
Background PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme.
Concentration 0.5 mg/ml
General Readings General / background references:Wagner LM et al. Effect of protein kinase Cgamma on gap junction disassembly in lens epithelial cells and retinal cells in culture. Mol Vis 8:59-66 (2002). PubMed PMID: 11951087
Dutil EM et al. Regulation of conventional protein kinase C isozymes by phosphoinositide-dependent kinase 1 (PDK-1). Curr Biol 8:1366-75 (1998). PubMed PMID: 9889098
Machide M et al. Selective activation of phospholipase C gamma1 and distinct protein kinase C subspecies in intracellular signaling by hepatocyte growth factor/scatter factor in primary cultured rat neocortical cells. J Neurochem 71:592-602 (1998). PubMed PMID: 9681449
Battaini F et al. Protein kinase C activity, translocation, and conventional isoforms in aging rat brain. Neurobiol Aging 16:137-48 (1995). PubMed PMID: 7777132
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
Aff - Purified
PKC alpha, iota, zeta (75%), beta 1, beta 2, epsilon and eta (63%) may cross-react in cells expressing high levels of these proteins.

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