discontinued

NSB975 PKC gamma antibody

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Rabbit anti Human PKC gamma

Product Description for PKC gamma

Rabbit anti Human PKC gamma.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PKC gamma

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic phosphopeptide derived from a region of human PKC gamma that contains threonine 674.
Application Western Blot, WB: Use at a concentration of 0.35 - 1.0 ug/ml. Detects a band of approximately 80 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., Western Blot
Background Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional grou(cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1.
Concentration 0.5 mg/ml
General Readings General / background references:Nguyen TA et al. LEDGF activation of PKC gamma and gap junction disassembly in lens epithelial cells. Exp Eye Res 76:565-72 (2003). PubMed PMID: 12697420
Aronowski J et al. Interplay between the gamma isoform of PKC and calcineurin in regulation of vulnerability to focal cerebral ischemia. J Cereb Blood Flow Metab 20:343-9 (2000). PubMed PMID: 10698072
Dutil EM et al. Regulation of conventional protein kinase C isozymes by phosphoinositide-dependent kinase 1 (PDK-1). Curr Biol 8:1366-75 (1998). PubMed PMID: 9889098
Toker A Signaling through protein kinase C. Front Biosci 3:D1134-47 (1998). PubMed PMID: 9792904
Format
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
PKC alpha (69%) may cross-react in cells expressing a high level of this protein. The peptide competition data (see Figure) suggest that this will cross-react with PKC alpha [pS657] (69% homologous), beta 1 [pS661] (54%) and PKC eta [pS674] (38%).
Species:
Human

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