NSB968 PKC iota + PKC lambda antibody

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Rabbit anti Human, Mouse PKC iota + PKC lambda

Product Description for PKC iota + PKC lambda

Rabbit anti Human, Mouse PKC iota + PKC lambda.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PKC iota + PKC lambda

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu, Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic phosphopeptide derived from a region of human PKC iota that contains threonine 555.
Application Western Blot, This antibody can be used in Western Blot where a 76 kDa band is seen. Recommended Starting Diluitons*: WB: 1/1000 *Optimal dilutions should be determined by the end user., Western Blot
Background Protein Kinase C iota (PKC iota) is a 76 kDa member of the atypical group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. Phosphorylation of PKC iota plays a key role in protein trafficking. Phosphorylated PKC iota is implicated in high glucose-induced T type II transforming growth factor-beta activity, TNF-alpha-stimulated NF-kappaB activation and Bcr/Abl mediated anti-apoptotic effect.
General Readings Tisdale EJ et al. Atypical protein kinase C plays a critical role in protein transport from pre-Golgi intermediates. J Biol Chem 278:38015-21 (2003). PubMed PMID: 12871960
Chuang LY et al. Regulation of type II transforming-growth-factor-beta receptors by protein kinase C iota. Biochem J 375:385-93 (2003). PubMed PMID: 12841849
Standaert ML et al. Skeletal muscle insulin resistance in obesity-associated type 2 diabetes in monkeys is linked to a defect in insulin activation of protein kinase C-zeta/lambda/iota. Diabetes 51:2936-43 (2002). PubMed PMID: 12351430
Selzer E et al. Protein kinase C isoforms in normal and transformed cells of the melanocytic lineage. Melanoma Res 12:201-9 (2002). PubMed PMID: 12140376
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
Sodium Azide
Immunogen affinity purified
Aff - Purified
This reacts with PKC lambda immunoprecipitates, indicating cross-reactivity for PKC lambda [pT563]. PKC zeta [pT560] (83% homologous) has been shown to cross-react by peptide competition. Peptide competition also suggests that this may partially cross-react with PKC beta-I [pS642] (58% homologous) and PKC gamma [pT655] (42% homologous).
Human, Mouse

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