NSB961 PKC mu/PKD specific pSer738/742 antibody

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0.1 ml / €390.00

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Rabbit anti Human, Mouse PKC mu/PKD specific

Product Description for PKC mu/PKD specific

Rabbit anti Human, Mouse PKC mu/PKD specific.
Properties: pSer738/742
Product is tested for Western blot / Immunoblot.

Properties for PKC mu/PKD specific

Product Category Primary Antibodies
Quantity 0.1 ml
Reactivity Hu, Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCm/PKD that contains serine 742. The sequence is conserved in mouse.
Property pSer738/742
Add. information Research Areas: Cellular Markers
Application Western Blot, This antibody is suitable for use in Western blotting. The recommended concentration for use in Western blotting is 1:1,000. *The optimal concentration should be determined for each specific application. , Western Blot
Background Protein Kinase Cm (PKCm), is a ~140 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKCm (also known as Protein Kinase D or PKD) is implicated in the regulation of multiple cellular processes including Golgi organization and membrane transport in epithelial cells. PKCm is phosphorylated on serine 742 (serine 748 for the mouse sequence) in the activation loop in a PKC-dependent pathway, mainly by PKCh and PKCe. This is critical for its catalytic activity, substrate phosphorylation and role in activating the ERK1 MAP Kinase signaling cascade.
General Readings Rykx, A., et al. (2003) Protein kinase D: a family affair. FEBS Lett. 546(1):81-86.
Storz, P. and A. Toker (2003) Protein kinase D mediates a stress-induced NF-kappaB activation and survival pathway. EMBO J. 22(1):109-120.
Waldron, R. D., et al. (2003) Protein kinase C phosphorylates protein kinase D activation loop Ser744 and Ser748 and releases autoinhibition by the pleckstrin homology domain. J. Biol. Chem. 278(1):154-163.
Brandlin, I., et al. (2002) Protein kinase C (PKC)eta-mediated PKC mu activation modulates ERK and JNK signal pathways. J. Biol. Chem. 277(8):6490-6496.
Hausser, A., et al. (2001) Protein kinase C mu selectively activates the mitogen-activated protein kinase (MAPK) p42 pathway. FEBS Lett. 492(1-2):39-44.
Vertommen, D., et al. (2000) Regulation of protein kinase D by multisite phosphorylation. Identification of phosphorylation sites by mass spectrometry and characterization by site-directed mutagenesis. J. Biol. Chem. 275(26):19567-19576.
Keranen, L.M., et al. (1995) Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. Curr. Biol. 5(12):1394-1403.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
PKC mu + PKD pecific [Ser738/Ser742]
Human, Mouse

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