NSB970 PKC theta antibody

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Rabbit anti Human PKC theta

Product Description for PKC theta

Rabbit anti Human PKC theta.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for PKC theta

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic phosphopeptide derived from a region of human PKC theta that contains serine 676.
Application Western Blot, Western Blot Detects a band of approximately 80 kDa. , Western Blot 0.35 - 1 ug/ml
Background Protein Kinase C theta (PKC theta) is an 80 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC theta plays a critical role in JNK activation, suggesting a role in the signaling processes of many biological responses such as inflammation and immune response, and also can play a specialized role in TCR mediated activation of T and B cells. The activation of PKC theta in T cells has been shown be linked to its recruitment to the membrane, and is mediated by PI3 kinase and Vav. PKC theta is a constitutively competent kinase and is autophosphorylated on serine 676 in the turn loop and serine 695 in the hydrophobic loop. Phosphorylation of serine 676 has minimal influence on the kinase activity of PKC theta but is believed to negatively regulate other PKC theta functions such as PKC theta mediated activation of NF kappa B.
Concentration 0.5 mg/ml
General Readings General / background references:Dennehy KM et al. Mitogenic signals through CD28 activate the protein kinase Ctheta-NF-kappaB pathway in primary peripheral T cells. Int Immunol 15:655-63 (2003). PubMed PMID: 12697665
Liu Y et al. Phosphorylation of the protein kinase C-theta activation loop and hydrophobic motif regulates its kinase activity, but only activation loop phosphorylation is critical to in vivo nuclear-factor-kappaB induction. Biochem J 361:255-65 (2002). PubMed PMID: 11772397
Gao T & Newton AC The turn motif is a phosphorylation switch that regulates the binding of Hsp70 to protein kinase C. J Biol Chem 277:31585-92 (2002). PubMed PMID: 12080070
Villalba M et al. Translocation of PKC[theta] in T cells is mediated by a nonconventional, PI3-K- and Vav-dependent pathway, but does not absolutely require phospholipase C. J Cell Biol 157:253-63 (2002). PubMed PMID: 11956228
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
Aff - Purified
This did not cross-react with any other PKC isoforms tested.

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