discontinued

DM3177 Plastin-L antibody

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0.1 mg / €280.00

Quick Overview

Mouse anti Human Plastin-L LPL4A.1

DM3177

Product Description for Plastin-L

Mouse anti Human Plastin-L LPL4A.1.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Plastin-L

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone LPL4A.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Human recombinant L-plastin protein.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blotting: Ab 1-2µg/ml for 2h at RT. Immunofluorescence: Fixed in methanol or 3% paraformaldehyde. Immunoprecipitation (Ref.2)(Native): Use Protein G; Ab 2µg/mg protein lysate. Recommended positive control: Human Polymorphonuclear Neutrophils and Monocytes. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Jones S L, Brown
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Format
State:
Purified
Specificity
Specificity:
Is a leukocyte specific actin bundling protein from the fibrin family of proteins. It has two actin-binding domains and two EF hand type calcium-binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L-plastin may play a role in regulating integrin mediated adhesion in leukocytes. L-plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosteronse in andrgen receptor positive prostate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene. Ab-1 does not react with T- or Iplastin1. Reacts with Human. Does not react with Mouse. Others not tested. Cellular Localization: cytoplasmic.

Mol. Wt of Antigen: 62kD

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