AP00567PU-N PLC1 antibody

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Rabbit anti Bovine, Human, Rat PLC1


Product Description for PLC1

Rabbit anti Bovine, Human, Rat PLC1.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for PLC1

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms DP3107, PLC-1
Presentation Purified
Reactivity Bov, Hu, Rt
Applications IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

A synthetic peptide derived from the C-terminus of Bovine PLCgamma1 protein.
Mol. Wt. of Antigen: 148 kDa
Application Immunoprecipitation (Denatured verified): Use Protein A; Ab 10 µg/mg protein lysate.
Western Blotting: Ab 5-10 µg/ml for 2 hrs at RT. Recommended positive control: Jurkat and A431 Cells.
Background Phospholipase C is a key enzyme in many signal transduction pathways stimulated by cytokines, hormones, growth factors. It hydrolyses phosphatidylinositol-4,5,-diphosphate into inositol-1,4,5,-triphosphate (IP3) and 1,2-diacylglycerol (DAG). IP3 is important for the release of Ca+2 from the intracellular reserves and DAG is involved in the activation of protein kinase C. There are ten different isoforms of PLC: PLCβ1-4, PLCγ1 and 2, PLCδ1-4 of which PLCγ1 is important for the tyrosine kinase dependent signaling through EGFR, PDGFR and TCR/CD3. It is ubiquitously expressed, while PLCγ2 is predominantly found in B lymphocytes. PLCγ1 has two SH2 domains and one SH3 domain. It binds to the auto tyrosine phosphorylated receptor through its SH2 domain and gets activated by tyrosine phosphorylation. PLCg1 is essential for the normal embryonic growth.
Concentration 1 mg/ml
General Readings
  1. Carpenter G, Qs Ji. (1999) Exp. Cell. Res. 253: 15-24.
  2. Kim M j, Kim e, ryu S H, Suh P G. (2000) 32:101-109.
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
Liquid IgG
This antibody detects Phospholipase Cγ1.
Cellular Localization: Cytoplasmic
Human, Rat, Cow.

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