AM03021SU-N PLSCR1 antibody

See related secondary antibodies

Search for all "PLSCR1"

0.1 ml / €240.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Rat PLSCR1 13A6 [TEC-23]

AM03021SU-N

More Views

  • AM03021SU-N

Product Description for PLSCR1

Mouse anti Rat PLSCR1 13A6 [TEC-23].
Presentation: Ascites

Properties for PLSCR1

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Phospholipid Scramblase 1
Presentation Ascites
Reactivity Rt
Clonality Monoclonal
Clone 13A6 [TEC-23]
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
BALB/c mice were immunized with pooled lipid rafts isolated from RBL-2H3 cells.
Hybridoma cells were obtained after fusion of SP02 mouse myeloma cells with spleen cells of immunized mice using standard procedures.
Application Immunoprecipitation.
Western Blotting: 1 μg/ml.
Flow Cytometry.
Immunocytochemistry.
Background PLSCR1 (Phospholipid Scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction.
Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.
Protocols Western Blot:
Positive control: RBL Rat basophilic leukemia cell line.
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% Triton X-100 in 20 mM Tris/Cl, 100 mM NaCl, 2 mM EDTA, 2 mM Na3Vo4, 10 mM glycerolphosphate, pH 8.0, 1 mM PMSF, 10 μg/ml aprotinin). Incubate 20 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min.
Note: Both reducing and non-reducing conditions.
Product Citations
Originator or purchased from resellers:
  • Smrz D, Lebduska P, Dráberová L, Korb J, Dráber P: Engagement of Phospholipid Scramblase 1 in Activated Cells: IMPLICATION FOR PHOSPHATIDYLSERINE EXTERNALIZATION AND EXOCYTOSIS. J Biol Chem. 2008 Apr 18;283(16):10904-18.
General Readings
  • Pastorelli C, Veiga J, Charles N, Voignier E, Moussu H, Monteiro RC, Benhamou M: IgE receptor type I-dependent tyrosine phosphorylation of phospholipid scramblase. J Biol Chem. 2001 Jun 8;276(23):20407-12. Epub 2001 Mar 20.
  • Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ: Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. Cancer Res. 2002 Jan 15;62(2):397-402.
  • Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH: Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J Virol. 2004 Sep;78(17):8983-93.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Preservatives:
15 mM Sodium Azide
State:
Liquid Undiluted Ascites
Ascites
Specificity
Specificity:
This antibody binds to rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa membrane-associated protein accelerating bidirectional movement of plasma membrane phospholipids during conditions of elevated calcium.
Species:
Rat.

Accessory Products

  • LinkedIn