discontinued

AP10352PU-N PMEPA1 / STAG1 antibody

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Rabbit anti Human PMEPA1 / STAG1

AP10352PU-N

Product Description for PMEPA1 / STAG1

Rabbit anti Human PMEPA1 / STAG1.
Presentation: Aff - Purified
Product is tested for Dot blot, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for PMEPA1 / STAG1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Solid tumor-associated 1 protein, TMEPAI, Transmembrane prostate androgen-induced protein
Presentation Aff - Purified
Reactivity Hu
Applications Dot, E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q969W9
Immunogen:
Purified TMEPA1 protein which was emulsified with adjuvants to achieve the desired antigenicity.
GeneID:
56937
Add. information Predicted Molecular Weight: 31 kDa
Application ELISA: 1/20,000-1/50,000.
Dot Blot: 1/20,000-1/50,000.
Western Blot: 1/500-1/1000.
Background The abnormal activation of the epidermal growth factor (EGF) pathway is one of the most common findings in human cancer, and a number of molecular devices of laboratory and clinical relevance have been designed to block this transduction pathway. The activation of 4 EGF receptor family members resulted in to large number of cellular events that might be regulating the metastasis and cell growth. The identification of new molecular targets working downstream of these pathways may provide new sites for therapeutic interventions for cancer diagnosis and potentially prevention and therapy. Several EGF target genes have been identified, one of them is Erg1.2 a mouse homolog of the solid tumor associated gene STAG1. Both in humans and in mice, it belongs to a new gene family that can give origin to several protein isoforms through alternative splicing and/or multiple translation starts. Sequence analysis and experimental data suggest that ERG1.2 is likely to function as a membrane-bound protein interacting with downstream signaling molecules through WW- and SH3-binding domains. Other members of this family include TMEPA1, and TPD52. PMEPA1 was identified originally as a highly androgen-inducible gene with prostate-abundant expression that was restricted to prostatic epithelial cells. PMEPA1 protein is a NEDD4 (ubiquitin-protein isopeptide ligase)-binding protein, which negatively regulates prostate cancer cell growth (1). During prostate cancer progression TMEPA1 gene transcription is reduced or lost suggesting a direct role of epigenetic events in this process.
PMEPA1 negatively regulates AR protein levels in different cell culture models. Transient expression of PMEPA1 down-regulates AR protein levels and AR transcriptional targets in prostate cancer cells. Conversely, knockdown of PMEPA1 leads to elevated levels of AR protein, AR transcriptional targets (prostate-specific antigen), and increased cell cycle S phase. The TMEPA1 mutant cells are impaired in NEDD4 recruitment shows attenuated AR ubiquitination and AR protein down-regulation.
Certain epigenetic cascade events contributes to the selective growth advantage during tumor progression. During prostate cancer progression the TMEPA1 gene is reduced or lost as a result of DNA methylation of SP1 sites within the PMEPA1 promoter may also contribute to the repression of PMEPA1 gene (2). The TMEPA1 negatively regulates the stability of AR protein by enhancing AR ubiquitination and proteosome-mediated degradation through NEDD4 and the TMEPA1-AR degradation pathway may represent a new androgen-dependent mechanism for regulating AR levels in prostate epithelial cells. The decrease in TMEPA1 in prostate cancers may lead to an increase in AR function and strengthen the biological role of TMEPA1 in prostate cancers. TMEPA1 is a 254 amino acid (apparent MW 30-31kDa) protein highly expressed in prostate cells.
Concentration 0.65-0.69 mg/ml (lot specific)
General Readings
  1. Li H, Xu LL, Masuda K, Raymundo E, McLeod DG, Dobi A, et al. A feedback loop between the androgen receptor and a NEDD4-binding protein, PMEPA1, in prostate cancer cells. J Biol Chem. 2008 Oct 24;283(43):28988-95. doi: 10.1074/jbc.M710528200. Epub 2008 Aug 14. PubMed PMID: 18703514. (Free PMC Article available, 6 images available)
  2. Richter E, Masuda K, Cook C, Ehrich M, Tadese AY, Li H, et al. A role for DNA methylation in regulating the growth suppressor PMEPA1 gene in prostate cancer. Epigenetics. 2007 Apr-Jun;2(2):100-9. Epub 2006 Dec 27. PubMed PMID: 18174752.
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography using immobilized antigen
Buffer System:
Stabilization buffer
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects a band of approximately 31 kDa.
Gene ID 56937

Accessory Products

Proteins and/or Positive Controls

Proteins for PMEPA1 / STAG1 (4 products)

Catalog No. Species Pres. Purity   Source  

PMEPA1 / STAG1 (transcript variant 4)

PMEPA1 / STAG1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

PMEPA1 / STAG1 (transcript variant 3)

PMEPA1 / STAG1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

PMEPA1 / STAG1

PMEPA1 / STAG1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PMEPA1 / STAG1

PMEPA1 / STAG1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for PMEPA1 / STAG1 (2 products)

Catalog No. Species Pres. Purity   Source  

PMEPA1 overexpression lysate

PMEPA1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

PMEPA1 overexpression lysate

PMEPA1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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