NB100-77673 PNAd antibody

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Rat anti Human, Mouse PNAd MECA-79


Product Description for PNAd

Rat anti Human, Mouse PNAd MECA-79.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for PNAd

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms HEV marker, High Endothelial Venules marker, Peripheral Node Addressin
Presentation Aff - Purified
Reactivity Hu, Ms
Applications C, IP, P, WB
Clonality Monoclonal
Clone MECA-79
Host Rat
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Mouse lymph node stromal cells
Application immunohistochemistry, immunoprecipitation, Western Blot, applications include: in vitro and in vivo blocking of cell adhesion, immunohistochemical staining of acetone-fixed frozen sections and 4% paraformaldehyde-fixed paraffin-embedded sections, the suggested use of this reagent is < 10 ug/ml. immunoprecipitation, and Western blotting., immunohistochemistry immunoprecipitation Western Blot
Background MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.
General Readings
  1. Streeter, P. R., et al., 1988. J. Cell Biol. 107: 1853.2. Berg, E. L., et al., 1993. Nature 366: 695.3. Hemmerich, S., et al., 1994
Storage Store at 4C. Do not freeze.
Immunogen affinity purified
Buffer System:
0.09% Sodium Azide
Immunogen affinity purified
Aff - Purified
PNAd (MECA-79)
Human, Mouse

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