DM3500 Podoplanin antibody

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0.2 ml / €370.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human Podoplanin 18H5

DM3500

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  • DM3500

Product Description for Podoplanin

Mouse anti Human Podoplanin 18H5.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Podoplanin

Product Category Primary Antibodies
Target Category
Quantity 0.2 ml
Synonyms Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
Presentation Supernatant
Reactivity Hu
Applications C, F, IP, P, WB
Clonality Monoclonal
Clone 18H5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q86YL7
GeneID:
10630
Application Flow Cytometry: 1/50.
Immunoprecipitation: 1/50.
Western blot: 1/100.
Immunohistochemistry on Frozen and Paraffin Embedded Sections: 1/50-1/200.
Background Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.
Product Citations
Purchased from Acris:
  1. Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, et al. Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1. Blood. 2007 Dec 15;110(13):4223-33. Epub 2007 Aug 30. PubMed PMID: 17761831.
  2. Riesenberg R, Weiler C, Spring O, Eder M, Buchner A, Popp T, et al. Expression of indoleamine 2,3-dioxygenase in tumor endothelial cells correlates with long-term survival of patients with renal cell carcinoma. Clin Cancer Res. 2007 Dec 1;13(23):6993-7002. PubMed PMID: 18056175.
  3. Conrad C, Niess H, Huss R, Huber S, von Luettichau I, Nelson PJ, et al. Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo. Circulation. 2009 Jan 20;119(2):281-9. doi: 10.1161/CIRCULATIONAHA.108.793208. Epub 2008 Dec 31. PubMed PMID: 19118255.
  4. Turner JD, Langley RS, Johnston KL, Gentil K, Ford L, Wu B, et al. Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis. J Biol Chem. 2009 Aug 14;284(33):22364-78. doi: 10.1074/jbc.M901528200. Epub 2009 May 19. PubMed PMID: 19458089. (Free PMC Article available, 9 images available)
  5. Birke K, Lütjen-Drecoll E, Kerjaschki D, Birke MT. Expression of podoplanin and other lymphatic markers in the human anterior eye segment. Invest Ophthalmol Vis Sci. 2010 Jan;51(1):344-54. doi: 10.1167/iovs.08-3307. Epub 2009 Sep 8. PubMed PMID: 19737877.
  6. Wendemagegn E, Tirumalae R, Böer-Auer A. Histopathological and immunohistochemical features of nodular podoconiosis. J Cutan Pathol. 2015 Mar;42(3):173-81. doi: 10.1111/cup.12441. Epub 2015 Feb 5. PubMed PMID: 25401490.
General Readings
  1. El-Chemaly S, Malide D, Zudaire E, Ikeda Y, Weinberg BA, Pacheco-Rodriguez G, et al. Abnormal lymphangiogenesis in idiopathic pulmonary fibrosis with insights into cellular and molecular mechanisms. Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3958-63. doi: 10.1073/pnas.0813368106. Epub 2009 Feb 23. PubMed PMID: 19237567. (Free PMC Article available, 5 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Concentrated Tissue Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Human. It does not react with Mouse.
Specificity
Specificity:
This antibody will detect Podoplanin on the surface of lymphatic endothelial cells and kidney podocytes by Immunostaining or Immunohistochemistry.
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for Podoplanin (7 products)

Catalog No. Species Pres. Purity   Source  

Podoplanin (transcript variant 3)

Podoplanin S. cerevisiae > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

Podoplanin (99-207, His-tag)

Podoplanin Human Purified > 95 % by SDS PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

Podoplanin (99-207, His-tag)

Podoplanin Human Purified > 95 % by SDS PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Podoplanin (Fragment)

Podoplanin Human Purified > 95 % by SDS-PAGE and visualised by silver stain E. coli
5 µg / €170.00
  Acris Antibodies GmbH

Podoplanin

Podoplanin Human 12.5% SDS-PAGE Stained with Coomassie Blue
10 µg / €380.00
  Abnova Taiwan Corp.

Podoplanin

Podoplanin Human 12.5% SDS-PAGE Stained with Coomassie Blue
25 µg / €510.00
  Abnova Taiwan Corp.

Podoplanin

SDS-Page: Podoplanin Protein [NBC1-18511] - PDPN, 13.4 kDa (130 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
0.1 mg / €380.00
  Novus Biologicals Inc.

Positive controls for Podoplanin (4 products)

Catalog No. Species Pres. Purity   Source  

PDPN overexpression lysate

PDPN overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.

PDPN overexpression lysate

PDPN overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.

PDPN overexpression lysate

PDPN overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.

Podoplanin Lysate

Western Blot: Podoplanin Lysate [NBL1-14262] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for PDPN.
0.1 mg / €400.00
  Novus Biologicals Inc.
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