SM1398 Poly-ADP-Ribose (PAR) antibody

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0.1 ml / €610.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Poly-ADP-Ribose (PAR) 10H

SM1398

Product Description for Poly-ADP-Ribose (PAR)

Mouse anti Human Poly-ADP-Ribose (PAR) 10H.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Poly-ADP-Ribose (PAR)

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms PADPR
Presentation Purified
Reactivity Hu
Applications C, IP, P, WB
Clonality Monoclonal
Clone 10H
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified poly (ADP-ribose) polymer, 10-50 unit chain length.
Isotype control AM03097PU-N
Application Western Blot (1/500).
Immunoprecipitation.
Immunohistology on Frozen/Paraffin Sections: This product requires protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.
Background PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).
Concentration 1.0 mg/ml
General Readings
  1. Kawamitsu H, Hoshino H, Okada H, Miwa M, Momoi H, Sugimura T. Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures. Biochemistry. 1984 Jul 31;23(16):3771-7. PubMed PMID: 6206890.
  2. Love S, Barber R, Wilcock GK. Increased poly(ADP-ribosyl)ation of nuclear proteins in Alzheimer's disease. Brain. 1999 Feb;122 ( Pt 2):247-53. PubMed PMID: 10071053.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Stabilizers:
50% Glycerol
State:
Liquid purified IgG fraction from Ascites
Purified
Species Reactivity
Species reactivity (expected):
Broad.
Species reactivity (tested):
Human.
Specificity
Specificity:
SM1398 recognises poly (ADP-ribose) modified proteins.
The antibody does not cross-react with RNA, DNA or monomers of ADP-ribose.

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