discontinued

NB100-80033 Polyethylene Glycol (PEG) antibody

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0.1 mg / €360.00

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Rabbit anti Polyethylene Glycol (PEG) PEG-B-47b

Product Description for Polyethylene Glycol (PEG)

Rabbit anti Polyethylene Glycol (PEG) PEG-B-47b.
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Polyethylene Glycol (PEG)

Product Category Primary Antibodies
Quantity 0.1 mg
Applications E, P, WB
Clonality Monoclonal
Clone PEG-B-47b
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
KLH-PEG with terminal methoxy group was used as an immunogen. This antibody recognizes the terminal methoxy group of the PEG molecule. Anti-PEG-47-Biotin is prepared by the biotinylation of the antibody via primary amines.
Application Western Blot, This antibody is useful for: Western Blot, ELISA, immunohistochemistry, Western Blot 15.6ng/mL
Background Polyethylene glycol(PEG)is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution (1). The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs (2,3). The modification of a biopharmaceutical with polyethylene glycol (PEG) increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions (4). As examples, PEG-adenosine deaminase (Adagen) is used for the treatment of severe combined immunodeficiency syndrome, PEGasparaginase (Oncaspar) is used for the treatment acute lymphoblastic leukemia, PEGinterferon alpha2a (Pegasys) is used for the treatment Hepatitis C, Branched PEGanti-VEGF aptamer (Pegaptanib, Macugen) is used for the treatment Macular degeneration(age-related) (5). An Anti-PEG antibody can be used to monitor a drugs pharmacokinetics, including distribution, metabolism and excretion. In addition, it can be used for the quality control of pegylated molecules in ELISA, WB and flow cytometery.
General Readings
  1. Guiotto A, Canevari M, Pozzobon M, Moro S, Orsolini P, Veronese FM. Anchimeric assistance effect on regioselective hydrolysis of branched PEGs: a mechanistic investigation. Bioorg Med Chem. 2004 Oct 1;12(19):5031-7. PubMed PMID: 15351387.
  2. Wong, S.S. (1991) Reactive groups of proteins and their modifying agents. In Chemistry of protein conjugation and cross-linking, p. 13, CRC Press
  3. Caliceti, P. et al.(1993) Active site protection of proteolytic enzymes by poly(ethylene glycol) surface modification. J. Bioact. Comp. Polym. 8,41-50
  4. Frank Leenders, celares GmbH, Berlin, Germany.(2006) PEGylation technology and biopharmaceuticals. Biopharmaceuticals. 6,39-40
  5. Francesco M.Veronese, Gianfranco Pasut. (2005) PEGylation, successful approach
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Label:
Biotin
Buffer System:
0.01% Sodium Azide
Specificity
Specificity:
KLH-PEG with terminal methoxy group was used as an immunogen. This recognizes the terminal methoxy group of the PEG molecule. Anti-PEG-47-Biotin is prepared by the biotinylation of the via primary amines.

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