AM26366LE-N Polymyxin B antibody

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0.2 mg / €410.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Polymyxin B 45

AM26366LE-N

Product Description for Polymyxin B

Mouse anti Polymyxin B 45.
Presentation: Low Endotoxin
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for Polymyxin B

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Low Endotoxin
Applications E, WB
Clonality Monoclonal
Clone 45
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Immunoassays.
Western blot: The typical starting working dilution is 1/10.
Background The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo.
Concentration > 0.2 mg/ml
General Readings
  1. Appelmelk BJ, Su D, Verweij-van Vught AM, Thijs BG, MacLaren DM. Polymyxin B-horseradish peroxidase conjugates as tools in endotoxin research. Anal Biochem. 1992 Dec;207(2):311-6. PubMed PMID: 1481986.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Preservatives:
0.02% Sodium Azide
State:
Liquid Culture Medium with a Low endotoxin Level
Low Endotoxin
Specificity
Specificity:
The monoclonal antibody 45 reacts with Polymyxin B. It enables the possibilities to study quantitatively the interaction of PMB and LPS.

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