AM32014PU-N POP7 antibody

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0.1 mg / €830.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human POP7 1F11

Product Description for POP7

Mouse anti Human POP7 1F11.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for POP7

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms RPP20, Ribonuclease P protein subunit p20, Ribonucleases P/MRP protein subunit POP7 homolog, hPOP7
Presentation Purified
Reactivity Hu
Applications E, WB
Clonality Monoclonal
Clone 1F11
Host Mouse
Isotype IgG2a
Molecular weight 20 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O75817
Immunogen:
Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
GeneID:
10248
Isotype control AM03096PU-N
Application ELISA.
Immunoblotting. 
Recommended Solvent: 100 mM PBS or Tris-HCl, pH 7.0
Background

The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli.
Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.

Caution

Be careful opening the vial since the antibody resides in a Vacuum.

Concentration 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%)
General Readings
  1. Welting TJ, Raijmakers R, Pruijn GJ. Autoantigenicity of nucleolar complexes. Autoimmun Rev. 2003 Oct;2(6):313-21. PubMed PMID: 14550872.
  2. van Eenennaam H, Jarrous N, van Venrooij WJ, Pruijn GJ. Architecture and function of the human endonucleases RNase P and RNase MRP. IUBMB Life. 2000 Apr;49(4):265-72. PubMed PMID: 10995027.
  3. Welting TJ, van Venrooij WJ, Pruijn GJ. Mutual interactions between subunits of the human RNase MRP ribonucleoprotein complex. Nucleic Acids Res. 2004 Apr 19;32(7):2138-46. Print 2004. PubMed PMID: 15096576. (Free PMC Article available, 8 images available)
Storage Store the antibody undiluted at 2-8°C or Add up to 0.05% Sodium Azide for long term storage.
Shelf life: One year from despatch.
Format
Buffer System:
10 mM Ammonium Bicarbonate buffer
Stabilizers:
2 mg BSA
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Dissolve in 200 μl buffer for a 0.5 mg/ml antibody concentration in 1% BSA
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:

Specificity has been tested in ELISA.
In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel).
Additional tests for cross reactivity have not yet been performed.

Gene ID 10248

Accessory Products

Proteins and/or Positive Controls

Proteins for POP7 (7 products)

Catalog No. Species Pres. Purity   Source  

POP7

POP7 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

POP7 (1-140, His-tag)

POP7 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €730.00
  OriGene Technologies GmbH

POP7 (1-140, His-tag)

POP7 Human Purified > 90 % by SDS - PAGE E. coli
20 µg / €300.00
  OriGene Technologies GmbH

POP7

POP7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

POP7

POP7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

POP7

POP7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

POP7

POP7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for POP7 (2 products)

Catalog No. Species Pres. Purity   Source  

POP7 293T Cell Transient Overexpression Lysate(Denatured)

POP7 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

POP7 overexpression lysate

POP7 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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