SP5299P PPAR alpha antibody

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Rabbit anti Mouse, Rat PPAR alpha


Product Description for PPAR alpha

Rabbit anti Mouse, Rat PPAR alpha.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for PPAR alpha

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Ms, Rt
Applications C, ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Synthetic Peptide: M(1) V D T E S P I C P L S P L E A D D(18) C corresponding to amino acid residues 1-18 from murine PPAR alpha.
A single amino acid substitution (I8 to L8) exists between the murine and human protein.
Application Western blot (1.0 µg/ml, detects an ~52 kDa protein representing PPAR alpha from 3T3-L1 cell lysate). Immunofluorescence (1/100, staining is observed predominantly in the nucleus as well the cytoplasm). Immunoprecipitation.
Successful use in immunohistochemistry (1/500) on frozen sections has been described for previous lots.
Background Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
Concentration 1.0 mg/ml
General Readings
  1. Neuroscience 131 (2005) 577-587)
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Epitope affinity chromatography.
Buffer System:
PBS containing 1 mg/ml BSA and 0.05% Sodium Azide as preservative.
Liquid purified IgG fraction.
Aff - Purified
SP5299P detects peroxisome proliferator activated receptor (PPAR) alpha from mouse tissues.

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