SP5300P PPAR delta antibody

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Rabbit anti Mouse PPAR delta


Product Description for PPAR delta

Rabbit anti Mouse PPAR delta.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Electrophoretic Mobility Shift Assay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for PPAR delta

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Ms
Applications C, EMSA, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Synthetic peptide corresponding to amino acid residues 1-14 from mouse PPAR delta.
AA Sequence:
M(1) E Q P Q E E T P E A R E E(14)C
This sequence is 86% conserved in human PPAR delta.
The immunizing peptide (SP5300CP) is available for use in neutralization and control experiments.
Add. information PPAR is also known as FAAR. The corresponding gene for the PPAR delta is NR1C2.
Application Western Blot: 1/500. Detects an ~49 kDa protein representing PPAR delta from NIH-3T3 cell lysate.
Immunohistochemistry on frozen sections: 1/200.
Immunofluorescence: 1/100.
Immunolocalization yields predominant nuclear staining.
Successful use in gel shift assay has been described for previous lots.
Background Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand.
Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and
WY-14, 643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR delta, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
Concentration 1.0 mg/ml
General Readings JBC Feb 2005 manuscript M412829200.
Neuroscience 131 (2005) 577-587.
Storage Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Epitope affinity chromatography
Buffer System:
PBS, containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative
Liquid IgG fraction
Aff - Purified
This antibody detects peroxisome proliferator activated receptor (PPAR) delta from tissues and cells. This antibody does not detect PPAR alpha or PPAR gamma.

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