TA319363 PPP1R13L (780-797) antibody

Rabbit polyclonal anti-iASSP antibody

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0.1 mg / €503.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Canine, Human, Mouse PPP1R13L

TA319363

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  • TA319363

Product Description for PPP1R13L

Rabbit anti Canine, Human, Mouse PPP1R13L.
Properties: (780-797)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for PPP1R13L

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13B-like protein, PPP1R13BL, RAI, RelA-associated inhibitor
Presentation Aff - Purified
Reactivity Can, Hu, Ms
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8WUF5
Immunogen:
Synthetic peptide corresponding to amino acids 780-797 of Human iASPP (isoform 1) protein
GeneID:
10848
Property 780-797
Application Suitable for use in ELISA, Immunohistochemistry and Western blot.
Recommended Dilutions:
ELISA: 1/40,000-1/160,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/1,000-1/5,000.
Background ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkappaB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.
Concentration 1.19 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Liu,Z.J., Lu,X. and Zhong,S. (2005) ASPP-Apoptotic specific regulator of p53. Biochim. Biophys. Acta 1756 (1), 77-80.
  2. Laska MJ, Nexø BA, Vistisen K, Poulsen HE, Loft S, Vogel U. Polymorphisms in RAI and in genes of nucleotide and base excision repair are not associated with risk of testicular cancer. Cancer Lett. 2005 Jul 28;225(2):245-51. PubMed PMID: 15885892.
  3. Zhang,X., Wang,M., Zhou,C., Chen,S. and Wang,J. (2005) The expression of iASPP in acute leukemias. Leuk. Res. 29 (2), 179-183.
  4. Slee EA, Gillotin S, Bergamaschi D, Royer C, Llanos S, Ali S, et al. The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization. Oncogene. 2004 Dec 2;23(56):9007-16. PubMed PMID: 15489900.
  5. Takada N, Sanda T, Okamoto H, Yang JP, Asamitsu K, Sarol L, et al. RelA-associated inhibitor blocks transcription of human immunodeficiency virus type 1 by inhibiting NF-kappaB and Sp1 actions. J Virol. 2002 Aug;76(16):8019-30. PubMed PMID: 12134007. (Free PMC Article available, 6 images available)
  6. Yang JP, Hori M, Sanda T, Okamoto T. Identification of a novel inhibitor of nuclear factor-kappaB, RelA-associated inhibitor. J Biol Chem. 1999 May 28;274(22):15662-70. PubMed PMID: 10336463.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse, Rat, Canine.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is directed against the iASPP protein.
Expect bands approximately 100 kDa and 50 kDa in size corresponding to Isoforms 1 and 2 respectively of iASPP protein by Western blotting in the appropriate cell lysate or extract.
Partial reactivity may occur against iASPP from Bovine.

Accessory Products

Proteins and/or Positive Controls

Proteins for PPP1R13L (4 products)

Catalog No. Species Pres. Purity   Source  

PPP1R13L

PPP1R13L Human Purified
  Abnova Taiwan Corp.

PPP1R13L

PPP1R13L Human Purified
  Abnova Taiwan Corp.

PPP1R13L

PPP1R13L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PPP1R13L

PPP1R13L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for PPP1R13L (1 products)

Catalog No. Species Pres. Purity   Source  

PPP1R13L 293T Cell Transient Overexpression Lysate(Denatured)

PPP1R13L 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
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