AM26220PU-N PR3 / C-ANCA antibody

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0.1 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human PR3 / C-ANCA PR3-G2

AM26220PU-N

Product Description for PR3 / C-ANCA

Mouse anti Human PR3 / C-ANCA PR3-G2.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay, Frozen Sections.

Properties for PR3 / C-ANCA

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms AGP7, Leukocyte proteinase 3, MBN, Myeloblastin, NP-4, Neutrophil proteinase 4, P29, PR-3, PRTN3, Proteinase 3, Wegener autoantigen
Presentation Purified
Reactivity Hu
Applications C, E, WB
Clonality Monoclonal
Clone PR3-G2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P24158
Immunogen:
A crude granule extract
GeneID:
5657
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western blot: The typical starting working dilution is 1:50.
Immunoassay.
Flow cytometry: The typical starting working dilution is 1:50.
Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
Background PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation, PR3 can cleave the PR3-ANCA complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin.
Concentration 0.1 mg/ml
General Readings
  1. Van Der Geld YM, Limburg PC, Kallenberg CG. Characterization of monoclonal antibodies to proteinase 3 (PR3) as candidate tools for epitope mapping of human anti-PR3 autoantibodies. Clin Exp Immunol. 1999 Dec;118(3):487-96. PubMed PMID: 10594572. (Free PMC Article available, 5 images available)
  2. Pederzoli M, Kantari C, Gausson V, Moriceau S, Witko-Sarsat V. Proteinase-3 induces procaspase-3 activation in the absence of apoptosis: potential role of this compartmentalized activation of membrane-associated procaspase-3 in neutrophils. J Immunol. 2005 May 15;174(10):6381-90. PubMed PMID: 15879139.
Storage

Store at 2 - 8 °C.
Shelf life: one year from despatch.

Format
Purification:
Protein G
Buffer System:
PBS
Preservatives:
0.02% sodium azide
Stabilizers:
0.1% bovine serum albumin
State:
Liquid 0.2 µm filtered Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
Monoclonal antibody PR3G-2 reacts with human proteinase 3 (PR3), a 30 kDa protein.

Accessory Products

Proteins and/or Positive Controls

Proteins for PR3 / C-ANCA (7 products)

Catalog No. Species Pres. Purity   Source  

PR3 / C-ANCA

PR3 / C-ANCA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PR3 / C-ANCA

PR3 / C-ANCA Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €399.00
  OriGene Technologies, Inc.

PR3 / C-ANCA

PR3 / C-ANCA Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

PR3 / C-ANCA

PR3 / C-ANCA Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

PR3 / C-ANCA

PR3 / C-ANCA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PR3 / C-ANCA

PR3 / C-ANCA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PR3 / C-ANCA

PR3 / C-ANCA Human Purified > 96 % Neutrophils
  BBI Solutions

Positive controls for PR3 / C-ANCA (3 products)

Catalog No. Species Pres. Purity   Source  

PR3 Lysate

Western Blot: PR3 Lysate [NBL1-14851] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PRTN3
  Novus Biologicals Inc.

PRTN3 293T Cell Transient Overexpression Lysate(Denatured)

PRTN3 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

PRTN3 overexpression lysate

PRTN3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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