AM32114PU-N PR3 / C-ANCA antibody

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0.1 mg / €620.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human PR3 / C-ANCA PR3G-2

AM32114PU-N

Product Description for PR3 / C-ANCA

Mouse anti Human PR3 / C-ANCA PR3G-2.
Presentation: Liquid
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay, Frozen Sections.

Properties for PR3 / C-ANCA

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms AGP7, Leukocyte proteinase 3, MBN, Myeloblastin, NP-4, Neutrophil proteinase 4, P29, PR-3, PRTN3, Proteinase 3, Wegener autoantigen
Presentation Liquid
Reactivity Hu
Applications C, E, WB
Clonality Monoclonal
Clone PR3G-2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P24158
Immunogen:
Immunization of mice with a crude granule extract.
GeneID:
5657
Application Flow Cytometry.
Immuinohistochemistry on frozen sections.
Western Blot.
Immunoassay. 
It is recommended that users test the reagent and determine their own optimal dilutions.
The typical starting working dilution is 1/50.
Background

PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener’s granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation, PR3 can cleave the PR3-ANCA complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst.
Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin

Concentration 0,1 mg/ml
Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Buffer System:
PBS
Preservatives:
0,02% sodium azide
Stabilizers:
0,1% BSA
State:
Liquid purified Ig fraction(0.2 µm filtered)
Liquid
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody reacts to Proteinase 3 (PR3)

Accessory Products

Proteins and/or Positive Controls

Proteins for PR3 / C-ANCA (7 products)

Catalog No. Species Pres. Purity   Source  

PR3 / C-ANCA

PR3 / C-ANCA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PR3 / C-ANCA

PR3 / C-ANCA Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €399.00
  OriGene Technologies, Inc.

PR3 / C-ANCA

PR3 / C-ANCA Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

PR3 / C-ANCA

PR3 / C-ANCA Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

PR3 / C-ANCA

PR3 / C-ANCA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PR3 / C-ANCA

PR3 / C-ANCA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PR3 / C-ANCA

PR3 / C-ANCA Human Purified > 96 % Neutrophils
  BBI Solutions

Positive controls for PR3 / C-ANCA (3 products)

Catalog No. Species Pres. Purity   Source  

PR3 Lysate

Western Blot: PR3 Lysate [NBL1-14851] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PRTN3
  Novus Biologicals Inc.

PRTN3 293T Cell Transient Overexpression Lysate(Denatured)

PRTN3 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

PRTN3 overexpression lysate

PRTN3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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