discontinued

BP8034 Procollagen Type III antibody

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0.1 ml / €550.00

Quick Overview

Rabbit anti Bovine, Human, Porcine Procollagen Type III

BP8034

Product Description for Procollagen Type III

Rabbit anti Bovine, Human, Porcine Procollagen Type III.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Radioimmunoassay, Paraffin Sections.

Properties for Procollagen Type III

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Collagen, Pro-Collagen
Presentation Purified
Reactivity Bov, Hu, Por
Applications C, E, ICC/IF, P, R
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified Human Procollagen type III.
Application RIA.
ELISA:
1/100-1/200.
Immunofluorescence (indirect, 1/40 on Frozen tissues).
Immunohistochemistry on Frozen and Paraffin Embedded Tissue Sections: 1/500.
Pretreatment: After removing Paraffin pre-treat with 0.2% Hyaluronidase (approx. 300 U/mg) in TBS, 15 min at 37°C, then block unspecific binding with blocking serum or 3% BSA in TBS and perform blocking of endogen Peroxidase with 1% H2O2 in TBS, if necessary.
Recommended Incubation Time: 60 min at room temperature or overnight at 2-8°C.
Positive Control: Human or Bovine Skin and Liver.
Background Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
Concentration 1.0 mg/ml
General Readings
  1. Trinchet JC, Hartmann DJ, Pateron D, Munz-Gotheil C, Callard P, Ville G, et al. Serum type I collagen and N-terminal peptide of type III procollagen in patients with alcoholic liver disease: relationship to liver histology. Alcohol Clin Exp Res. 1992 Apr;16(2):342-6. PubMed PMID: 1590556.
  2. Farjanel J, Hartmann DJ, Guidet B, Luquel L, Offenstadt G. Four markers of collagen metabolism as possible indicators of disease in the adult respiratory distress syndrome. Am Rev Respir Dis. 1993 May;147(5):1091-9. PubMed PMID: 8484615.
Storage Store the antibiody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography.
Buffer System:
PBS without BSA or preservatives.
State:
Lyophilized purified Ig fraction.
Purified
Reconstitution:
Restore with 0.5 ml distilled water.
Specificity
Specificity:
Recognizes Human and bovine Pro-Collagen Type III (100%).
Human and Bovine Collagene Type I < 0.1% in RIA at 1/200 dilution.
Reacts also with Porcine Pro-Collagen Type III.
Species:
Human, Bovine and Porcine.

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