BP8034S Procollagen Type III antibody

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0.1 ml / €410.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Bovine, Human, Porcine Procollagen Type III


Product Description for Procollagen Type III

Rabbit anti Bovine, Human, Porcine Procollagen Type III.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Radioimmunoassay.

Properties for Procollagen Type III

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Collagen, Pro-Collagen
Presentation Purified
Reactivity Bov, Hu, Por
Applications C, E, ICC/IF, P, R
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified Human Procollagen type III C-terminal and N-terminal PIIICP separated.
Application RIA: > 1/200.
ELISA: 1/100-1/200 (OD ≥ 500). 
Immunofluorescence (indirect, 1/40 on Frozen tissues).
Immunohistochemistry on Frozen and Paraffin Embedded Tissue Sections: 1/500.
Pretreatment: After removing Paraffin pre-treat with 0.2% Hyaluronidase (approx. 300 U/mg) in TBS, 15 min at 37°C, then block unspecific binding with blocking serum or 3% BSA in TBS and perform blocking of endogen Peroxidase with 1% H2O2 in TBS, if necessary.
Incubation Time: 60 min at room temperature or overnight at 2-8°C.
Positive Control: Human or Bovine Skin and Liver.
Background Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
Concentration ~1.0 mg/ml (after reconstitution)
Product Citations
Purchased from Acris:
  1. Fronczek J, Lulf R, Ibrahim Korkmaz H, Witte BI, van de Goot FR, Begieneman MP, et al. Analysis of morphological characteristics and expression levels of extracellular matrix proteins in skin wounds to determine wound age in living subjects in forensic medicine. Forensic Sci Int. 2015 Jan;246:86-91. doi: 10.1016/j.forsciint.2014.11.013. Epub 2014 Nov 22. PubMed PMID: 25485947.
General Readings
  1. Trinchet JC, Hartmann DJ, Pateron D, Munz-Gotheil C, Callard P, Ville G, et al. Serum type I collagen and N-terminal peptide of type III procollagen in patients with alcoholic liver disease: relationship to liver histology. Alcohol Clin Exp Res. 1992 Apr;16(2):342-6. PubMed PMID: 1590556.
  2. Farjanel J, Hartmann DJ, Guidet B, Luquel L, Offenstadt G. Four markers of collagen metabolism as possible indicators of disease in the adult respiratory distress syndrome. Am Rev Respir Dis. 1993 May;147(5):1091-9. PubMed PMID: 8484615.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
PBS without BSA or preservatives
Lyophilized purified Ig fraction
Restore with 0.1 ml sterile distilled water.
Human and Bovine Pro-Collagen Type III (100%).
Human and Bovine Pro-Collagen Typ I and Collagen Typ I <0.1% (RIA at 1/200 dilution).
Human, Bovine (and Porcine).

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