AM05312PU-N Progesterone receptor antibody

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0.1 mg / €580.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Equine, Human, Porcine Progesterone receptor hPRa2 + hPRa3

Product Description for Progesterone receptor

Mouse anti Equine, Human, Porcine Progesterone receptor hPRa2 + hPRa3.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Progesterone receptor

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR
Presentation Purified
Reactivity Eq, Hu, Por
Applications C, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone hPRa2 + hPRa3
Host Mouse
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06401
Immunogen:
Hybridoma produced by the fusion of splenocytes from RBF/DN mice immunized with PgR from a human endometrial carcinoma (EnCa 101) grown in athymic mice and mouse myeloma HL-1 Friendly cells.
GeneID:
5241
Application Western Blot: 1 µg/ml.
Immunoprecipitation: 2 µg/mg of protein lysate.
Immunohistochemistry on Frozen and Paraffin Sections: 2-4 µg/ml.
Immunofluorescense. 
Positive Control: T47D or breast carcinoma.
Background Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. This antibody is excellent for immunohistochemical staining of formalin/paraffin tissues.
Concentration Lot specific
General Readings
  1. Satyaswaroop PG. Development of a preclinical model for hormonal therapy of human endometrial carcinomas. Ann Med. 1993 Apr;25(2):105-11. PubMed PMID: 8489746.
  2. Shyamala G, Schweitzer M, Ullrich SJ. Relationship between 90-kilodalton heat shock protein, estrogen receptor, and progesterone receptor in human mammary tumors. Breast Cancer Res Treat. 1993;26(1):95-100. PubMed PMID: 8400328.
  3. Satyaswaroop PG, Clarke CL, Zaino RJ, Mortel R. Apparent resistance in human endometrial carcinoma during combination treatment with tamoxifen and progestin may result from desensitization following downregulation of tumor progesterone receptor. Cancer Lett. 1992 Feb 29;62(2):107-14. PubMed PMID: 1540937.
  4. Clarke CL, Graham J, Roman SD, Sutherland RL. Direct transcriptional regulation of the progesterone receptor by retinoic acid diminishes progestin responsiveness in the breast cancer cell line T-47D. J Biol Chem. 1991 Oct 5;266(28):18969-75. PubMed PMID: 1918012.
  5. Schneider W, Ramachandran C, Satyaswaroop PG, Shyamala G. Murine progesterone receptor exists predominantly as the 83-kilodalton 'A' form. J Steroid Biochem Mol Biol. 1991 Mar;38(3):285-91. PubMed PMID: 2009220.
  6. Mortel R, Zaino RJ, Satyaswaroop PG. Designing a schedule of progestin administration in the control of endometrial carcinoma growth in the nude mouse model. Am J Obstet Gynecol. 1990 Apr;162(4):928-34; discussion 934-6. PubMed PMID: 2327463.
  7. Clarke CL, Roman SD, Graham J, Koga M, Sutherland RL. Progesterone receptor regulation by retinoic acid in the human breast cancer cell line T-47D. J Biol Chem. 1990 Jul 25;265(21):12694-700. PubMed PMID: 2373707.
  8. Clarke CL. Cell-specific regulation of progesterone receptor in the female reproductive system. Molecular and Cellular Endocrinology, 1990 May 7, 70(3):C29- 33.
  9. Satyaswaroop PG, Mortel R. Hormonal treatment of endometrial carcinoma: an overview and new development in biology. J Steroid Biochem Mol Biol. 1990 Dec 20;37(6):997-1001. PubMed PMID: 2285611.
  10. Shyamala G, Schneider W, Schott D. Developmental regulation of murine mammary progesterone receptor gene expression. Endocrinology. 1990 Jun;126(6):2882-9. PubMed PMID: 2190799.
  11. Alexander IE, Clarke CL, Shine J, Sutherland RL. Progestin inhibition of progesterone receptor gene expression in human breast cancer cells. Mol Endocrinol. 1989 Sep;3(9):1377-86. PubMed PMID: 2608065.
  12. Feil PD, Clarke CL, Satyaswaroop PG. A polyclonal antiserum against the rabbit progesterone receptor recognizes the human receptor: biochemical characterization. Cell Biochem Funct. 1989 Apr;7(2):139-46. PubMed PMID: 2766471.
  13. Satyaswaroop PG, Zaino RJ, Mortel R. Carcinoma of the endometrium and hormonal receptors. Cancer Treat Res. 1989;49:71-92. PubMed PMID: 2577204.
  14. Tabibzadeh SS, Satyaswaroop PG. Sex steroid receptors in lymphoid cells of human endometrium. Am J Clin Pathol. 1989 Jun;91(6):656-63. PubMed PMID: 2658542.
  15. Zaino RJ, Clarke CL, Feil PD, Satyaswaroop PG. Differential distribution of estrogen and progesterone receptors in rabbit uterus detected by dual immunofluorescence. Endocrinology. 1989 Nov;125(5):2728-34. PubMed PMID: 2676490.
  16. Feil PD, Clarke CL, Satyaswaroop PG. Progesterone receptor structure and protease activity in primary human endometrial carcinoma. Cancer Res. 1988 Mar 1;48(5):1143-7. PubMed PMID: 3277707.
  17. Feil PD, Clarke CL, Satyaswaroop PG. Progestin-mediated changes in progesterone receptor forms in the normal human endometrium. Endocrinology. 1988 Nov;123(5):2506-13. PubMed PMID: 2971526.
  18. Zaino RJ, Clarke CL, Mortel R, Satyaswaroop PG. Heterogeneity of progesterone receptor distribution in human endometrial adenocarcinoma. Cancer Res. 1988 Apr 1;48(7):1889-95. PubMed PMID: 3280122.
  19. Clarke CL, Feil PD, Satyaswaroop PG. Progesterone receptor regulation by 17 beta-estradiol in human endometrial carcinoma grown in nude mice. Endocrinology. 1987 Nov;121(5):1642-8. PubMed PMID: 3665838.
  20. Satyaswaroop PG; Zaino R; Clarke CL; Mortel R. Nude mouse system in the study of tumor biology, treatment strategies and progesterone receptor physiology in human endometrial carcinoma. Journal of Steroid Biochemistry, 1987, 27(1-3):431-8.
  21. Satyaswaroop PG, Zaino RJ, Mortel R. Steroid receptors and human endometrial carcinoma: studies in a nude mouse model. Cancer Metastasis Rev. 1987;6(3):223-41. PubMed PMID: 3690801.
  22. Clarke CL, Zaino RJ, Feil PD, Miller JV, Steck ME, Ohlsson-Wilhelm BM, et al. Monoclonal antibodies to human progesterone receptor: characterization by biochemical and immunohistochemical techniques. Endocrinology. 1987 Sep;121(3):1123-32. PubMed PMID: 3304978.
  23. Gray GO, Satyaswaroop PG. Species crossreactivity of human progesterone receptor monoclonal antibodies: Western blot analysis. Biochem Biophys Res Commun. 1988 Dec 30;157(3):1067-77. PubMed PMID: 2462866.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
PBS containing 0.08% Sodium Azide as preservative
Purity:
Purified
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody equally recognizes two proteins: 116kDa (triplet) and 81kDa (singlet), identified as the hormone-binding high (B) and low (A) MW forms of human PgR.
Species:
Human, Horse and Pig.
Gene ID 5241
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for Progesterone receptor (7 products)

Catalog No. Species Pres. Purity   Source  

Progesterone receptor

Progesterone receptor Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Progesterone receptor

Progesterone receptor Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Progesterone receptor

Progesterone receptor Human
  Abnova Taiwan Corp.

Positive controls for Progesterone receptor (1 products)

Catalog No. Species Pres. Purity   Source  

PGR overexpression lysate

PGR overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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