AM32853PU-T Progesterone receptor antibody

See related secondary antibodies

Search for all "Progesterone receptor"

20 µg / €180.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Progesterone receptor PR484

Product Description for Progesterone receptor

Mouse anti Human Progesterone receptor PR484.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Frozen Sections, Paraffin Sections.

Properties for Progesterone receptor

Product Category Primary Antibodies
Target Category
Quantity 20 µg
Synonyms NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR
Presentation Purified
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone PR484
Host Mouse
Isotype IgG1
Molecular weight PR-A (81kDa) and PR-B (116kDa).
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06401
Immunogen:
Recombinant Human Progesterone Receptor protein.
GeneID:
5241
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot: 0.5-1 µg/ml for 2 hours at RT. 
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: T47-D Cells or Breast Cancers.
Background The progesterone receptor (also known as NR3C3) is a member of the steroid or nuclear hormone receptor superfamily family of proteins. Family members are involved in diverse physiological functions including embryonic development, cell differentiation, and homeostasis. The receptors function by binding to DNA in the nucleus and regulating the transcription of target genes. The progesterone receptor is activated by its ligand progesterone, which is a steroid hormone involved in the menstrual cycle, pregnancy and embryogenesis. Progesterone receptor expression status is commonly used as a marker for classifying breast cancers and for predicting prognosis and response to endocrine therapy. That is, antibody positive versus antibody negative tumors can have potential implications with respect to predicted outcome, treatment, or disease monitoring. Progesterone receptor is expressed as two major isoforms, PR-A (81 kDa) and PR-B (116 kDa). These isoforms are generated from alternative transcriptional start sites within the progesterone receptor gene. Both forms are ligand activated by progesterone although they differ in their relative ability to activate target gene transcription. Antibody to progesterone receptor is widely used to study breast and other progesterone receptor containing tissues such as the endometrium. Researchers are encouraged to consult the scientific literature for additional information regarding the use of antibody to measure progesterone receptor as part of assessing breast or other tumor types.
Concentration 0.2 mg/ml
General Readings
  1. Mote PA, Johnston JF, Manninen T, Tuohimaa P, Clarke CL. Detection of progesterone receptor forms A and B by immunohistochemical analysis. J Clin Pathol. 2001 Aug;54(8):624-30. PubMed PMID: 11477119. (Free PMC Article available, 2 images available)
  2. Mylonas I, Makovitzky J, Friese K, Jeschke U. Immunohistochemical labelling of steroid receptors in normal and malignant human endometrium. Acta Histochem. 2009;111(4):349-59. doi: 10.1016/j.acthis.2008.11.012. Epub 2009 Feb 4. PubMed PMID: 19195687.
  3. Cserni G, Francz M, Kálmán E, Kelemen G, Komjáthy DC, Kovács I, et al. Estrogen receptor negative and progesterone receptor positive breast carcinomas-how frequent are they? Pathol Oncol Res. 2011 Sep;17(3):663-8. doi: 10.1007/s12253-011-9366-y. Epub 2011 Jan 26. PubMed PMID: 21267685.
  4. Press M, Spaulding B, Groshen S, Kaminsky D, Hagerty M, Sherman L, et al. Comparison of different antibodies for detection of progesterone receptor in breast cancer. Steroids. 2002 Aug;67(9):799-813. PubMed PMID: 12123792.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal antibody is specific to Progesterone Receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone.
It is excellent for immunohistochemical staining of formalin/paraffin tissues.
Cellular Localization: Nucleus.
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for Progesterone receptor (7 products)

Catalog No. Species Pres. Purity   Source  

Progesterone receptor

Progesterone receptor Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Progesterone receptor

Progesterone receptor Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Progesterone receptor

Progesterone receptor Human
  Abnova Taiwan Corp.

Positive controls for Progesterone receptor (1 products)

Catalog No. Species Pres. Purity   Source  

PGR overexpression lysate

PGR overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
  • LinkedIn